When one looks at a specimen with a light microscope, how does one know what is real and what is an illusion created by the apparatus itself? Light and Video Microscopy presents the step-by-step journey through philosophy, psychology, and particularly geometrical and physical optics involved in interpreting images formed by light microscopes. This knowledge addresses the intricacies setting up light microscopes that allow one to visualize transparent specimens and, in the process, quantitatively determine various physico-chemical properties of the specimen. One is empowered to use past, present, and future microscopical techniques to visualize the microscopic world and find the real nature of life in the image.
This third edition of Light and Video Microscopy has been fully revised and updated to include the most recent developments in microscopy, ensuring that it continues to be the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy guides the reader to the most appropriate method for their specific scenario, and then guides them through the accurate interpretation of the image. With the presentation of geometrical optics, it assists the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems.
This book will be valuable for anyone using a light or video microscope to identify and understand living and developing cells.
- Fully revised and updated to include ability to image three-dimensional (3D) structures, measure interactions by multicolor colocalization, and record dynamic processes in living cells at the nanometer scale
- New appendices on Diffraction theory and Advanced Image Processing
- Provides practical application, lab exercises and case studies of the mathematics, physics, and biology used in microscopy
- Discusses bright field, dark field, phase-contrast, fluorescence, interference, differential interference and modulation contrast microscops and oblique illumination, photomicrography
2. Geometrical Optics
3. Physical Optics
4. Bright Field Microscopy
5. Phase Contrast and Dark Field Microscopy, Rheinberg and Oblique Illumination
7. Fluorescence Microscopy
8. Polarization Microscopy
9. Interference Microscopy
10. Differential Interference Microscopy
11. Modulation Contrast Microscopy
12. Video and Digital Microscopy
13. Image Processing and Analysis
14. Various Kinds of Microscopes and Accessories
Randy O. Wayne is a plant cell biologist at Cornell University notable for his work on plant development. In particular, along with his colleague Peter K. Hepler, Wayne established the powerful role of calcium in regulating plant growth; accordingly, their 1985 article, Calcium and plant development, was cited by at least 405 subsequent articles to earn the "Citation Classic" award from Current Contents magazine and has been cited by hundreds more since 1993. He is an authority on how plant cells sense gravity through pressure, on the water permeability of plant membranes, light microscopy, as well as the effects of calcium on plant development. He has published over 50 articles and is the author of another book, Light and Video Microscopy.