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Light and Video Microscopy. Edition No. 3

  • Book
  • June 2019
  • Elsevier Science and Technology
  • ID: 4700367

Light and Video Microscopy, Third Edition provides a step-by-step journey through philosophy, psychology and the geometrical and physical optics involved in interpreting images formed by light microscopes. The book addresses the intricacies necessary to set up light microscopes that allow one to visualize transparent specimens and, in the process, quantitatively determine various physico-chemical properties of specimens. This updated edition includes the most recent developments in microscopy, ensuring that it continues to be the most comprehensive, easy-to-use, and informative guide on light microscopy. With its presentation of geometrical optics, it assists the reader in understanding image formation and light movement within the microscope.

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Table of Contents

1. The Relation Between the Object and the Image
2. Geometrical Optics
3. Physical Optics
4. Bright Field Microscopy
5. Phase Contrast and Dark Field Microscopy, Rheinberg and Oblique Illumination
6. Photomicrography
7. Fluorescence Microscopy
8. Polarization Microscopy
9. Interference Microscopy
10. Differential Interference Microscopy
11. Modulation Contrast Microscopy
12. Video and Digital Microscopy
13. Image Processing and Analysis
14. Various Kinds of Microscopes and Accessories


Randy O. Wayne Plant cell biologist at Cornell University. Randy O. Wayne is a plant cell biologist at Cornell University notable for his work on plant development. In particular, along with his colleague Peter K. Hepler, Wayne established the powerful role of calcium in regulating plant growth; accordingly, their 1985 article, Calcium and plant development, was cited by at least 405 subsequent articles to earn the "Citation Classic" award from Current Contents magazine and has been cited by hundreds more since 1993. He is an authority on how plant cells sense gravity through pressure, on the water permeability of plant membranes, light microscopy, as well as the effects of calcium on plant development. He has published over 50 articles and is the author of another book, Light and Video Microscopy.