Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Labelling, Vol 622

  • ID: 4720960
  • Book
  • 412 Pages
  • Elsevier Science and Technology
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Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in this series on enzymology
  • Updated release includes the latest information on methods to measure ubiquitin chain length and linkage, genetic approaches to study the yeast ubiquitin system, amongst many other timely topics
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1. Design of optogenetic proteins David S. Lawrence 2. Application of optogenetic proteins David S. Lawrence 3. Antibody aggregation mechanism probed by a fluorescently-labeled antibody with fluorescence correlation spectroscopy Shinya Honda 4. Bimane labeling of ß-arrestins to measure their interaction with GPCRs Arun K. Shukla 5. Reversible biotinylation of proteins for investigating their interaction with partners Arun K. Shukla 6. Chemical biology approaches to study RNA cytidine acetylation Jordan Meier 7.. Salt sensitive intein in robotic production of peptides Shih-Che Sue 8. Isotope-coded Mass Spec Tags to study interfacial protein-membrane interactions Nicole S. Sampson 9. Direct Cloning by Transformation-Associated Recombination and Heterologous Expression of Biosynthetic Gene Cluster Bradley Moore 10. Allosteric protein switches: from rational design to real applications John Karanicolas 11. Recombinant expression of protein kinases and their use for synthetic biology Stefan T. Arold 12. Small molecule target identification using photo-affinity chromatography Timothy W. Corson 13. Studying protein interactions by in-cell NMR Alexander Shekhtman 14. Investigating the role of glycosylation in protein structure, function, and stability Zhongping Tan 15. Drug inducible genome editing Yu Wang 16. Drug inducible genome activation Yu Wang 17. Computation biology towards directed biosynthesis of natural peptide products Shiou-Chuan (Sheryl) Tsai 18. Emerging protein engineering approaches for imaging applications Itaru Hamachi 19. Engineering structure and function of glycosyltransferases Marcelo E. Guerin 20. Using Cas-Targeted m6A reader proteins to study the effects of reader proteins on single RNAs Bryan C. Dickinson 21. Synthetic genetic circuits for regulation of sexual reproduction Shinya Honda
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