Basic Biotechnique for Bioprocess and Bioentrepreneurship deals with the entire field of industrial biotechnology, starting from basic laboratory techniques, to scale-up, process development, demonstration and commercialization. The book compiles currently scattered materials on the topic and updates information based on practical experience and requirements.
- Offers guidance for readers on how to develop new microbiological and biotechnical processes
- Focuses on solutions to practical difficulties at all levels, creating a thorough understanding of bioprocess development, commercialization, technology transfer, and more
- Provides multifaceted coverage, with industry insights from leaders in the field
- Gives solutions to practical difficulties at all levels, i.e., Lab, Scale-Up and the Commercial stage
UNIT-I: ISOLATION, SCREENING AND CULTURE MAINTENANCE 1. Isolation of microbes 2. Screening strategies 3. Identification: Morphological, Biochemical and genetic characterization 4. Activity enhancing strategies: Enrichment and Statistical method 5. Culture maintenance, preservation and improvement
UNIT-II: LABORATORY TECHNIQUES & INSTRUMENTATION 6. Biomolecules: Types, Homogenization, Bead beater, sonication 7. Centrifugation: Basic principle, types 8. Spectroscopy: Basic principle types and uses 9. Protein Purification: Basic Principle and techniques 10. Chromatography: Basic principle, types and applications 11. Electrophoresis: Basic principle, types and applications
UNIT-III: GENOMIC AND PROTEOMIC ANALYSIS 12. DNA, RNA, isolation methods, primer designing, sequence submission, phylogenetic analysis 13. RDT and genetic engineering: basic of RDT method, PCR and application 14. Protein sequence analysis 15. Protein structure prediction 16. Docking strategies 17. Methods of protein and drug interaction
UNIT-IV: INDUSTRIALLY IMPORTANT ENZYMES 18. Types of enzymes and their properties 19. Enzyme kinetics 20. Important industrial enzymes: Basic information, Assay and application
UNIT-V: TECHNIQUES IN PROCESS DEVELOPMENT 21. Basic Fermentation 22. Sterilization 23. Laboratory and scale-up 24. Bioprocess: Control, management and Biosafety issues 25. Demonstration and industrial scale up 26. Downstream Processing 27. Waste Management and Environment
UNIT-VI: FEASIBILITY, MARKETING AND BIOBUSINESS 28. Bio-business opportunitie 29. Feasibility and Business Plan 30. Patenting, IPR and Regulatory Issues 31.Technology Transfer 32. Bio-product marketing strategies
Dr. Arvind Kumar Bhatt has Ph.D. in Life Sc. with outstanding achievements in Academics, R&D, Industry and State Govt. departments where he has worked for more than last 26 years.. He is also Member of several Professional organizations, Editorial Board of Scientific Journals and has more than 40 research papers to his credit published in Scientific Journals of repute. He has guided/ guiding students for Ph. D. Degrees in Biotechnology/Microbiology/Life Sciences.
Bhatia, Ravi Kant
Bhalla, Tek Chand