Basic Biotechniques for Bioprocess and Bioentrepreneurship

Table of Contents

Part I: Isolation, screening and culture maintenance 1. Isolation of microorganisms 2. Screening strategies 3. Identification, morphological, biochemical, and genetic characterization of microorganisms 4. Microbial activity and productivity enhancement strategies 5. Culture maintenance, preservation, and strain improvement

Part II: Laboratory techniques and instrumentation 6. Biomolecules: Types, homogenization, bead beater, sonication 7. Centrifugation: Basic principle, types 8. Spectroscopy-Principle, types, and applications 9. Protein purification: Basic principles and techniques 10. Chromatography: Basic principle, types, and applications 11. Electrophoresis: Basic principle, types, and applications

Part III: Genomic and proteomic analysis 12. DNA, RNA isolation, primer designing, sequence submission, and phylogenetic analysis 13. RDT and genetic engineering: Basic of RDT method, PCR, and application 14. Protein sequence analysis 15. Computational strategies and tools for protein tertiary structure prediction 16. Docking strategies 17. A beginner's guide to measuring binding affinity during biomolecular interactions

Part IV: Industrially important enzymes 18. Enzymes and their significance in the industrial bioprocesses 19. Enzyme kinetics: Industrially important enzymes 20. Industrial enzymes: Basic information, assay, and applications

Part V: Techniques in process development 21. Fundamentals of fermentation technology 22. Sterilization in bioprocesses 23. Scale-up: Lab to commercial scale 24. Bioprocess: Control, management, and biosafety issues 25. Demonstration and industrial scale-up 26. Downstream processing of biotechnology products 27. Waste management and environment

Part VI: Feasibility, marketing and biobusiness 28. Biobusiness opportunities 29. Feasibility analysis and business plan 30. Patenting, IPR, and regulatory issues 31. Commercialization and technology transfers of bioprocess 32. Bioproduct marketing strategies

Authors

Arvind Kumar Bhatt Department of Biotechnology, Himachal Pradesh University, India. Dr. Arvind Kumar Bhatt has Ph.D. in Life Sc. with outstanding achievements in Academics, R&D, Industry and State Govt. departments where he has worked for more than last 26 years.. He is also Member of several Professional organizations, Editorial Board of Scientific Journals and has more than 40 research papers to his credit published in Scientific Journals of repute. He has guided/ guiding students for Ph. D. Degrees in Biotechnology/Microbiology/Life Sciences. Ravi Kant Bhatia Department of Biotechnology, Himachal Pradesh University, India. Tek Chand Bhalla Department of Biotechnology, Himachal Pradesh University, India.