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Tumor Immunology and Immunotherapy - Molecular Methods, Vol 629. Methods in Enzymology

  • ID: 4790368
  • Book
  • November 2019
  • 552 Pages
  • Elsevier Science and Technology
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Tumor Immunology and Immunotherapy - Molecular Methods, Volume 629, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this release include Droplet digital PCR for measuring circulating tumor-derived DNA, Detection and quantification of cytosolic DNA, Methods to detect endogenous dsRNA induction and recognition, Quantification of eIF2alpha phosphorylation during immunogenic cell death, Assessment of annexin A1 release during immunogenic cell death, Luciferase-assisted detection of extracellular ATP in the course of ICD, The P2X7 receptor: structure and function, and much more.

  • Contains the authority of authors who are leaders in their field
  • Provides a comprehensive source on new methods and research in enzymology

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1. Detection of circulating tumor DNA (ctDNA) by digital droplet polymerase chain reaction (dd-PCR) in liquid biopsies

Sara Valpione and Luca Campana

2. Detection and quantification of cytosolic DNA

Sheila Spada, Takahiro Yamazaki and Claire Vanpouille-Box

3. Methods to detect endogenous dsRNA induction and recognition

Ilias Ettayebi, Helen Loo Yau and Daniel D. De Carvalho

4. Quantification of eIF2alpha phosphorylation during immunogenic cell death

Lucillia Bezu, Alejandra Wu Chuang, Juliette Humeau, Guido Kroemer and Oliver Kepp

5. Assessment of annexin A1 release during immunogenic cell death

Elisa Elena Baracco, Adriana Petrazzuolo and Guido Kroemer

6. Luciferase-assisted detection of extracellular ATP and ATP metabolites during immunogenic death of cancer cells

George R. Dubyak

7. Quinacrine-mediated detection of intracellular ATP

Sabrina Forveille, Juliette Humeau, Allan Sauvat, Lucillia Bezu, Guido Kroemer and Oliver Kepp

8. Structure, function and techniques of investigation of the P2X7 receptor (P2X7R) in mammalian cells

Francesco Di Virgilio, Lin-Hua Jiang, Sèbastien Roger, Simonetta Falzoni, Alba Clara Sarti, Valentina Vultaggio-Poma, Paola Chiozzi and Elena Adinolfi

9. LEGENDplexT: Bead-assisted multiplex cytokine profiling by flow cytometry

Jason S. Lehmann, Priyanka Rughwani, Melissa Kolenovic, Shaoquan Ji and Binggang Sun

10. Methods for measuring HMGB1 release during immunogenic cell death

Liwei Zhao, Peng Liu, Oliver Kepp and Guido Kroemer

11. Different measures of HMGB1 location in cancer immunology

Carolina Mendonça Gorgulho, Pranav Murthy, Lance Liotta, Virginia Espina and Michael T. Lotze

12. Reliable detection of indoleamine 2,3 dioxygenase-1 in murine cells and tissues

Sunil Thomas, Lisa Laury-Kleintop and George C. Prendergast

13. Quantification of IDO1 enzyme activity in normal and malignant tissues

Lijie Zhai, Erik Ladomersky, April Bell, Corey Dussold, Krislyn Cardoza, Jun Qian, Kristen L. Lauing and Derek A. Wainwright

14. Enzyme activity of circulating CD73 in human serum

Silvana Morello, Roberta Turiello, Gabriele Madonna, Aldo Pinto, Paolo A. Ascierto and Mariaelena Capone

15. Measurement of CD73 enzymatic activity using luminescence-based and colorimetric assays

Bertrand Allard, Isabelle Cousineau, Kathleen Spring and John Stagg

16. Distinguishing perforin-mediated lysis and granzyme-dependent apoptosis

Jesse A. Rudd-Schmidt, Joseph A. Trapani and Ilia Voskoboinik

17. Elucidating the role of an immunomodulatory protein in cancer: From protein expression to functional characterization

Leepakshi Khurana, Mei ElGindi, Pathricia V. Tilstam and Georgios Pantouris

18. In vitro assay for the development of small molecule inhibitors targeting PD-1/PD-L1

Wenjie Zhai, Xiuman Zhou, Jiangfeng Du and Yanfeng Gao

19. Crystallographic approaches to study the interaction modes of PD-1- and CTLA-4-blocking antibodies

Norimichi Nomura, Yayoi Nomura, Yumi Sato and So Iwata

20. High-throughput discovery of cancer-targeting TCRs

Lukas Bunse, Edward W. Green and Michael Platten

21. TCR validation toward gene therapy for cancer

Edward W. Green, Lukas Bunse, Matthias Bozza, Khwab Sanghvi and Michael Platten

22. Analysis of TCR ß CDR3 sequencing data for tracking anti-tumor immunity

Jiajia Zhang, Zhicheng Ji and Kellie N. Smith

23. Quantitative analysis of the T cell receptor repertoire

Imran Uddin, Annemarie Woolston, Thomas Peacock, Kroopa Joshi, Mazlina Ismail, Tahel Ronel, Connor Husovsky and Benny Chain

24. CRATES: A one-step assembly method for Class 2 CRISPR arrays

Chunyu Liao, Rebecca A. Slotkowski and Chase L. Beisel
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