Tumor Immunology and Immunotherapy - Molecular Methods, Volume 629, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this release include Droplet digital PCR for measuring circulating tumor-derived DNA, Detection and quantification of cytosolic DNA, Methods to detect endogenous dsRNA induction and recognition, Quantification of eIF2alpha phosphorylation during immunogenic cell death, Assessment of annexin A1 release during immunogenic cell death, Luciferase-assisted detection of extracellular ATP in the course of ICD, The P2X7 receptor: structure and function, and much more.
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Table of Contents
1. Detection of circulating tumor DNA (ctDNA) by digital droplet polymerase chain reaction (dd-PCR) in liquid biopsiesSara Valpione and Luca Campana
2. Detection and quantification of cytosolic DNA
Sheila Spada, Takahiro Yamazaki and Claire Vanpouille-Box
3. Methods to detect endogenous dsRNA induction and recognition
Ilias Ettayebi, Helen Loo Yau and Daniel D. De Carvalho
4. Quantification of eIF2alpha phosphorylation during immunogenic cell death
Lucillia Bezu, Alejandra Wu Chuang, Juliette Humeau, Guido Kroemer and Oliver Kepp
5. Assessment of annexin A1 release during immunogenic cell death
Elisa Elena Baracco, Adriana Petrazzuolo and Guido Kroemer
6. Luciferase-assisted detection of extracellular ATP and ATP metabolites during immunogenic death of cancer cells
George R. Dubyak
7. Quinacrine-mediated detection of intracellular ATP
Sabrina Forveille, Juliette Humeau, Allan Sauvat, Lucillia Bezu, Guido Kroemer and Oliver Kepp
8. Structure, function and techniques of investigation of the P2X7 receptor (P2X7R) in mammalian cells
Francesco Di Virgilio, Lin-Hua Jiang, S?bastien Roger, Simonetta Falzoni, Alba Clara Sarti, Valentina Vultaggio-Poma, Paola Chiozzi and Elena Adinolfi
9. LEGENDplexT: Bead-assisted multiplex cytokine profiling by flow cytometry
Jason S. Lehmann, Priyanka Rughwani, Melissa Kolenovic, Shaoquan Ji and Binggang Sun
10. Methods for measuring HMGB1 release during immunogenic cell death
Liwei Zhao, Peng Liu, Oliver Kepp and Guido Kroemer
11. Different measures of HMGB1 location in cancer immunology
Carolina Mendon?a Gorgulho, Pranav Murthy, Lance Liotta, Virginia Espina and Michael T. Lotze
12. Reliable detection of indoleamine 2,3 dioxygenase-1 in murine cells and tissues
Sunil Thomas, Lisa Laury-Kleintop and George C. Prendergast
13. Quantification of IDO1 enzyme activity in normal and malignant tissues
Lijie Zhai, Erik Ladomersky, April Bell, Corey Dussold, Krislyn Cardoza, Jun Qian, Kristen L. Lauing and Derek A. Wainwright
14. Enzyme activity of circulating CD73 in human serum
Silvana Morello, Roberta Turiello, Gabriele Madonna, Aldo Pinto, Paolo A. Ascierto and Mariaelena Capone
15. Measurement of CD73 enzymatic activity using luminescence-based and colorimetric assays
Bertrand Allard, Isabelle Cousineau, Kathleen Spring and John Stagg
16. Distinguishing perforin-mediated lysis and granzyme-dependent apoptosis
Jesse A. Rudd-Schmidt, Joseph A. Trapani and Ilia Voskoboinik
17. Elucidating the role of an immunomodulatory protein in cancer: From protein expression to functional characterization
Leepakshi Khurana, Mei ElGindi, Pathricia V. Tilstam and Georgios Pantouris
18. In vitro assay for the development of small molecule inhibitors targeting PD-1/PD-L1
Wenjie Zhai, Xiuman Zhou, Jiangfeng Du and Yanfeng Gao
19. Crystallographic approaches to study the interaction modes of PD-1- and CTLA-4-blocking antibodies
Norimichi Nomura, Yayoi Nomura, Yumi Sato and So Iwata
20. High-throughput discovery of cancer-targeting TCRs
Lukas Bunse, Edward W. Green and Michael Platten
21. TCR validation toward gene therapy for cancer
Edward W. Green, Lukas Bunse, Matthias Bozza, Khwab Sanghvi and Michael Platten
22. Analysis of TCR ? CDR3 sequencing data for tracking anti-tumor immunity
Jiajia Zhang, Zhicheng Ji and Kellie N. Smith
23. Quantitative analysis of the T cell receptor repertoire
Imran Uddin, Annemarie Woolston, Thomas Peacock, Kroopa Joshi, Mazlina Ismail, Tahel Ronel, Connor Husovsky and Benny Chain
24. CRATES: A one-step assembly method for Class 2 CRISPR arrays
Chunyu Liao, Rebecca A. Slotkowski and Chase L. Beisel
