Genome Engineering via CRISPR-Cas9 System

Table of Contents

1. An introduction to genome editing CRISPR-Cas systems2. Evolution and mechanism of CRISPR-Cas9 systems3. Exploring the potential of CRISPR-Cas9 for removal of human viruses4. Programmable removal of bacterial pathogens using CRISPR-Cas9 system5. Targeted genome editing using CRISPR-Cas9 system in fungi6. CRISPR-Cas9 system for fungi genome engineering towards industrial applications7. Development and challenges of using CRISPR-Cas9 system in mammalians8. CRISPR technology for cell line development and engineering9. Exploring the potential of genome editing CRISPR-Cas9 system in cancer 10. CRISPR-Cas9 for therapy: the challenges and ways to overcome them11. Engineered Cas9 nucleases12. The current progress of CRISPR/Cas9 development in plants13. Fruit crops improvement using CRISPR-Cas9 system14. CRISPR-Cas9 engineered viral immunity in plants15. Genome engineering in medicinally important plants using CRISPR/Cas9 tool16. Genome editing of algal species by CRISPR Cas9 for biofuels17. Development and use of CRISPR in industrial applications18. CRISPR interference based gene silencing in bacteria: Advantages and limitations19. Genome engineering in insects: focus on the CRISPR/Cas9 system20. Recent Progress of CRISPR- Cas9 in Zebra Fish21. CRISPR: a revolutionary tool for genome engineering in the protozoan parasites22. Biosafety, biosecurity, patents, and regulatory issues

Authors

Vijai Singh Department of Biosciences, School of Science, Indrashil University, Rajpur, Mehsana, India. Dr Vijai Singh is an Associate Professor and Dean (Research & Innovation) at School of Sciences, Indrashil University, Rajpur, Mehsana, Gujarat, India. He was an Assistant Professor in the Department of Biological Sciences and Biotechnology at the Institute of Advanced Research, Gandhinagar, India and also an Assistant Professor in the Department of Biotechnology at the Invertis University, Bareilly, India. Prior to that, he was a Postdoctoral Fellow in the Synthetic Biology Group at the Institute of Systems and Synthetic Biology, Paris, France and School of Energy & Chemical Engineering at the Ulsan National Institute of Science and Technology, Ulsan, South Korea. He received his Ph.D. in Biotechnology (2009) from the National Bureau of Fish Genetic Resources, Uttar Pradesh Technical University, Lucknow, India with a research focus on the development of molecular and immunoassays for diagnosis of Aeromonas hydrophila. His research interests are focused on building novel biosynthetic pathways for production of medically and industrially important biomolecules. Additionally, his laboratory is working on CRISPR-Cas9 tools for genome editing. He has more than 8 years of research and teaching experience in synthetic biology, metabolic engineering, bioinformatics, microbiology, and industrial microbiology. He has published 79 articles, 51 chapters, 15 books and 4 patents. He serves as an associate editor, editorial board member, and reviewer of several peer-reviewed journals. He is also a member of the Board of Study and Academic Council of Indrashil University and is the Member Secretary of the Institutional Biosafety Committee (IBSC) at the same University. Pawan K. Dhar School of Biotechnology, Jawaharlal Nehru University, New Delhi, India. Prof. Pawan K. Dhar is the Dean, School of Biotechnology, Jawaharlal Nehru University, New Delhi and heads an active synthetic biology group. Prior to this he held scientific positions at RIKEN Institute, Keio University and Kyoto University (Japan) Bioinformatics Institute, (Singapore) and Manipal University (India). Prof. Dhar received his Ph.D. in 1993 from Banaras Hindu University (Varanasi) for his work on Human Genetics. Prof Dhar's lab holds specialization in making functional proteins from non-expressing DNA sequences leading to the development of a potentially groundbreaking drug discovery platform. Prof. Dhar serves in the external board of referees for European Science Foundation and Indian Government science funding agencies.