Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others.
Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields.
- Provides basic understanding and a clear picture on how to design, use and implement the CRISPR-Cas9 system in different organisms
- Explains how to create an animal model for disease research and screening purposes using CRISPR
- Discusses the application of CRISPR-Cas9 systems in basic sciences, biomedicine, virology, bacteriology, molecular biology, neurology, cancer, industry, and many more
1. An introduction to genome editing CRISPR-Cas systems 2. Evolution and mechanism of CRISPR-Cas9 systems 3. Exploring the potential of CRISPR-Cas9 for removal of human viruses 4. Programmable removal of bacterial pathogens using CRISPR-Cas9 system 5. Targeted genome editing using CRISPR-Cas9 system in fungi 6. CRISPR-Cas9 system for fungi genome engineering towards industrial applications 7. Development and challenges of using CRISPR-Cas9 system in mammalians 8. CRISPR technology for cell line development and engineering 9. Exploring the potential of genome editing CRISPR-Cas9 system in cancer 10. CRISPR-Cas9 for therapy: the challenges and ways to overcome them 11. Engineered Cas9 nucleases 12. The current progress of CRISPR/Cas9 development in plants 13. Fruit crops improvement using CRISPR-Cas9 system 14. CRISPR-Cas9 engineered viral immunity in plants 15. Genome engineering in medicinally important plants using CRISPR/Cas9 tool 16. Genome editing of algal species by CRISPR Cas9 for biofuels 17. Development and use of CRISPR in industrial applications 18. CRISPR interference based gene silencing in bacteria: Advantages and limitations 19. Genome engineering in insects: focus on the CRISPR/Cas9 system 20. Recent Progress of CRISPR- Cas9 in Zebra Fish 21. CRISPR: a revolutionary tool for genome engineering in the protozoan parasites 22. Biosafety, biosecurity, patents, and regulatory issues
Dr. Vijai Singh is an Associate Professor in the Department of Biosciences, School of Science at Indrashil University, Mehsana, Gujarat, India. Prior to this, Dr. Singh was a postdoctoral fellow at Institute of Systems and Synthetic Biology, France and School of Energy and Chemical Engineering at Ulsan National Institute of Science and Technology, South Korea. Dr. Singh has served as an Assistant Professor in the Department of Biotechnology at Invertis University, India and School of Biological Sciences and Biotechnology, Institute of Advanced Research, India. Dr Singh received his Ph.D. in Biotechnology from Dr. APJ Abdul Kalam Technical University / National Bureau of Fish Genetic Resources, Lucknow, India with a research focus on the development of molecular and immunoassay for Aeromonas hydrophila. Dr. Singh has designed and characterized a number of synthetic oscillators, gene networks, lycopene pathway, MAGE and CRISPR-Cas9 system in Escherichia coli. Currently, Dr. Singh's laboratory focuses on the construction of a novel biosynthetic pathway for the production of pigments, chemicals, and fuels. Additionally, Dr. Singh's laboratory works on developing CRISPR-based platform for disease diagnosis and eradication of MDR pathogens. Dr. Singh has published 72 articles in the peer-reviewed journals, 19 chapters, and 1 book. Dr. Singh serves as a member of the editorial board and reviewer of a number of peer-reviewed journals.
Dhar, Pawan K.
Prof. Pawan K. Dhar is the Dean, School of Biotechnology, Jawaharlal Nehru University, New Delhi and heads an active synthetic biology group. Prior to this he held scientific positions at RIKEN Institute, Keio University and Kyoto University (Japan) Bioinformatics Institute, (Singapore) and Manipal University (India). Prof. Dhar received his Ph.D. in 1993 from Banaras Hindu University (Varanasi) for his work on Human Genetics. Prof Dhar's lab holds specialization in making functional proteins from non-expressing DNA sequences leading to the development of a potentially groundbreaking drug discovery platform. Prof. Dhar serves in the external board of referees for European Science Foundation and Indian Government science funding agencies.