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Tumor Immunology and Immunotherapy - Cellular Methods Part B, Vol 632

  • ID: 4829354
  • Book
  • 412 Pages
  • Elsevier Science and Technology
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Tumor Immunology and Immunotherapy - Cellular Methods Part B, Volume 632, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered include Quantitation of calreticulin exposure associated with immunogenic cell death, Side-by-side comparisons of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death, Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometry, Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies, Methods to assess DC-dependent priming of T cell responses by dying cells, and more.

  • Contains content written by authorities in the field
  • Provides a comprehensive view on the topics covered
  • Includes a high level of detail
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1. Quantitation of calreticulin exposure associated with immunogenic cell death Peng Liu 2. Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death Jitka Fucikova 3. Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometry Guido Kroemer and Oliver Kepp 4. Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies Ilio Vitale 5. Methods to assess DC-dependent priming of T cell responses by dying cells Jitka Fucikova 6. Monitoring tumor antigen cross-presentation with human dendritic cells purified from the blood Anne Hosmalin 7. Assessing tumor antigen cross-presentation from live tumor cells Anne Hosmalin 8. Improved method to isolate mouse macrophages and DCs from the gut Arthur Mortha 9. Standardized isolation of mouse and human macrophages from the blood and bone marrow Jeffrey William Pollard 10. Methods to study tumor-associated and iPS-derived macrophages Jeffrey William Pollard 11. In vitro assessment of cancer cell-induced polarization of macrophages Karin E. de Visser 12. Protocol to assess the suppression of T-cell proliferation by human MDSC Pierre van der Bruggen 13. Enzymatic and cellular assays to quantify the suppressive activity of MDSCs Vincenzo Bronte 14. Functional Assay to Assess T-cell Inhibitory Properties of Myeloid Derived Suppressor Cells (MDSCs) Isolated from the Tumor Microenvironment of murine Glioma Models Maria G. Castro 15. FACS Isolation and Analysis of Human Circulating and Tumor Neutrophils Jeffrey William Pollard 16. Flow cytometry-based isolation of tumor-associated regulatory T cells and assessment of their suppressive potential Karin E. de Visser 17. Assessment of lipid load in tumor-infiltrating Tregs by flow cytometry Silvia Piconese 18. Preparation of single cells from tumors for single-cell RNA sequencing Dmitriy Zamarin 19. Characterization of the tumor infiltrate by multiparametric flow cytometry and unbiased high dimensional data analysis Romina Goldszmid 20. Phenotyping of tumor infiltrating immune cells using mass-cytometry (CyTOF) Jérôme Galon 21. Isolation and Characterization of Immune Cells from the Tumor Microenvironment of Genetically Engineered Pediatric High Grade Glioma Models Using the Sleeping Beauty Transposon System Maria G. Castro 22. Analysis of the immune status from peripheral whole blood with a single-tube multicolor flow cytometry assay Udo S. Gaipl 23. Ex vivo assessment of in vivo antibody targeting to immune cells in preclinical models Niroshana Anandasabapathy 24. Flow cytometry-based assessment of direct-targeting anti-cancer antibody immune effector functions Olivera J. Finn 25. Enzyme-linked immunospot assay to monitor antigen-specific cellular immune responses in mouse tumor models Laura Bracci 26. High-throughput analysis of cell-cell crosstalk in ad hoc designed microfluidic chips for oncoimmunology applications Giovanna Schiavoni 27. Generation of affinity ranged antigen-expressing tumor cell lines Pedro Romero 28. Methods to quantify immunogenicity in tumor-derived exosomes sheila spada
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