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Tumor Immunology and Immunotherapy - Integrated Methods Part A, Vol 635. Methods in Enzymology

  • ID: 4844359
  • Book
  • 412 Pages
  • Elsevier Science and Technology
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Tumor Immunology and Immunotherapy - Integrated Methods Part A, Volume 635 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Specific chapters to this release include Deconvolution of the immunological contexture of mouse tumors with multiplexed immunohistochemistry, High-dimensional multiplexed immunohistochemical characterization of immune contexture in human cancers, Multiplex assay by IHC for melanoma tumor microenvironment evaluation, Characterization of the tumor immune microenvironment by multispectral image analysis of multiplex immunofluorescence images, Phenotyping of immune cells in situ using multispectral imaging quantification, and much more.

  • Authored by leaders in the field of enzymology
  • Provides a comprehensiveness level of discussion on the field
  • Presents a highly specialized group of topics that delve deep into new updates and future prospects
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1. Deconvolution of the immunological contexture of mouse tumors with multiplexed immunohistochemistry 2. High-dimensional multiplexed immunohistochemical characterization of immune contexture in human cancers 3. Multiplex assay by IHC for melanoma tumor microenviroment evaluation 4. Characterization of the tumor immune microenvironment by multispectral image analysis of multiplex immunofluorescence images 5. Phenotyping of immune cells in situ using multispectral imaging quantification 6. Multiplex immunohistochemistry as a novel tool for the topographic assessment of the bone marrow stem cell niche 7. Immunoscore assay for the immune classification of solid tumors: technical aspects, improvements and clinical perspectives 8. Tissue transcriptome-based computation algorithms for determining immune cell compositions in tumor microenvironment 9. Deconvoluting tumor-infiltrating immune cells from RNA-seq data using quanTIseq 10. DIY: Visualizing the immune landscape of tumors using transcriptome and methylome data 11. A comprehensive approach to gene expression profiling in immune cells 12. Using epigenetic data to estimate immune composition in admixed samples 13. Concurrent in situ analysis of point mutations and immune infiltrate in FFPE cancers 14. Immunohistochemistry for DAMP and autophagy detection

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