Organoids are complex three-dimensional in vitro organ-like model systems. Organoids can be derived from pluripotent stem cells or primary donor tissue and have been used to address fundamental questions about development, stem cell biology and organ regeneration. Human organoids have invigorated new exploration into the cellular makeup of human organs during development, in the adult and during disease. Efforts to improve complexity in organoid systems, and to make organoid systems more robust, reproducible and controlled have prompted continued refinement of methods used to generate and culture organoids. This volume will highlight recent and emerging advances that describe organoid differentiation protocols for many different organ systems, that implement organoids as tools to understand complexity and maturation, high content drug screening, disease modeling, and understanding development and evolution. Readers can expect to gain knowledge in the most recent differentiation protocols from experts in their respective areas.
- The first comprehensive collection of pluripotent stem cell derived organoid protocols
- Cutting edge methods
- Methods to generate organoids from many organ systems