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Human Pluripotent Stem Cell Derived Organoid Models, Vol 159. Methods in Cell Biology

  • ID: 4911861
  • Book
  • July 2020
  • Region: Global
  • 312 Pages
  • Elsevier Science and Technology
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Organoids are complex three-dimensional in vitro organ-like model systems. Organoids can be derived from pluripotent stem cells or primary donor tissue and have been used to address fundamental questions about development, stem cell biology and organ regeneration. Human organoids have invigorated new exploration into the cellular makeup of human organs during development, in the adult and during disease. Efforts to improve complexity in organoid systems, and to make organoid systems more robust, reproducible and controlled have prompted continued refinement of methods used to generate and culture organoids. This volume will highlight recent and emerging advances that describe organoid differentiation protocols for many different organ systems, that implement organoids as tools to understand complexity and maturation, high content drug screening, disease modeling, and understanding development and evolution. Readers can expect to gain knowledge in the most recent differentiation protocols from experts in their respective areas.

  • The first comprehensive collection of pluripotent stem cell derived organoid protocols
  • Cutting edge methods
  • Methods to generate organoids from many organ systems
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1. Pluripotent stem cell derived gastric organoids Yana Zavros 2. Pluripotent stem cell derived esophageal organoids James Wells 3. Pluripotent stem cell derived small intestinal organoids David R. Hill 4. Pluripotent stem cell derived colonic organoids Jorge Munera 5. Pluripotent stem cell intestinal organoids with an Enteric Nervous System Maxime Mahe 6. Pluripotent stem cell derived airway organoids Finn Hawkins 7. Pluripotent stem cell derived alveolar organoids Shimpei Gotoh 8. Pluripotent stem cell derived liver bud organoids Takanori Takebe 9. Pluripotent stem cell derived thyroid organoids Sabine Costagliola 10. Pluripotent stem cell derived renal organoids Jennifer Harder 11. Pluripotent stem cell derived cerebral organoids Gray Camp 12. Generating ventral spinal organoids from human induced pluripotent stem cells Shi Yan Ng 13. Pluripotent stem cell derived retinal organoids Jason Meyer 14. Pluripotent stem cell derived inner ear organoids Eri Hashino 15. Pluripotent stem cell derived skin Karl R. Koehler 16. Pluripotent stem cell derived cholangiocytes and cholangiocyte Organoids Anne Dubart-Kupperschmitt
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