Eosinophil Ultrastructure: Atlas of Eosinophil Cell Biology and Pathology focuses on eosinophils and their functional roles in inflammation, host defense and normal homeostatic activities. The book explores the ultrastructure of human eosinophils, highlighting biological processes observed in normal, experimental and pathological conditions. Created to fill a void in the eosinophil literature, this book has an extensive array of electron microscopic images that illustrate the diversity of eosinophil morphology. While the atlas is primarily a learning and teaching tool, it also acts as a helpful resource for researchers who want to identify distinguishing features and structural changes that arise during studies of human eosinophils.
- Helps readers understand human eosinophils in studies focused on structural biology, cellular immunology, innate and adaptive immunity, immune responses to pathogens, immunopathology and inflammatory responses
- Provides a core of essential knowledge to identify both immature and mature eosinophils
- Comprises a representative compilation of the eosinophil ultrastructure during biological processes, such as activation and degranulation, observed mostly in experimental conditions
- Highlights eosinophil biological processes found in vivo during human diseases, thus providing a link between basic science and clinical aspects
- Helps identify distinguishing features and structural changes that arise during studies of human eosinophils in culture
The Cell Biology of Human Eosinophils Sections Aspects to be explored 1.1 General Morphology 1.1.1 Immature Eosinophils Early eosinophilic myelocyte Late eosinophilic myelocyte Eosinophilic myelocyte in mitosis 1.1.2 Mature Eosinophils General view Morphology of typical organelles and structures in high magnification: secretory granules, lipid bodies (LBs), Eosinophil Sombrero Vesicles (EoSVs); Views in intact cells and after isolation Cell surface morphology/ CD9 immunolabeling Multivesicular bodies (MVBs) Golgi (normal and Brefeldin A-disrupted) Peripheral Endoplasmic Reticulum (ER) Glycogen granules Organelle interactions: LB-ER; EoSV-granule 1.2 Eosinophil Biological Processes 1.2.1 Activation Increased number of LBs, specific granules changes (number and/or structure), increased number of EoSVs, shape changes 1.2.2 Migration 1.2.3 Secretion/Degranulation Constitutive secretion Classical exocytosis Piecemeal degranulation Cytolytic degranulation Secretion of extracellular vesicles Images from stimulus-induced secretion: A23187, CCL5, CCL11, INF-g, TNF-a, SCF, IgE Immuno-EM for cytokines, tetraspanins and cationic proteins Diaminobenzidina stained eosinophils 1.2.4 Vesicular Trafficking Structural aspects of the vesicular system EoSVs Immuno-EM for 5-LO, CD63, STX17, IL-4, IL-4R, PDI 1.2.5 Cell Death Apoptosis, Necrosis (Cytolysis), EETosis EOSINOPHIL ULTRASTRUCTURE
Selected Eosinophil-Associated Diseases Sections Aspects to be explored: biological processes shown above will be highlighted during human diseases. 2.1 Allergies 2.2 Gastrointestinal disorders Inflammatory Bowel Disease Crohn's disease 2.3 Hypereosinophilic syndrome (HES) 2.4 Skin disorders 2.5 Tumors
Rossana Melo is a Professor of Cell Biology and Principal Investigator at Federal University of Juiz de Fora (UFJF), Brazil. She earned her MSc and PhD degrees in Cell Biology from the Federal University of Minas Gerais (UFMG), Brazil, and received postdoctoral training from Harvard Medical School, USA. Rossana Melo serves on a number of research committees and programs and has been a distinct Brazilian Researcher at the Conselho Nacional de Desenvolvimento Científico e Tecnológico -CNPq (National Research Council). She is the leader of the Cell Biology Research Group (UFJF/CNPq) and a visiting Scientist/Professor at Beth Israel Deaconess Medical Center/Harvard University. Her areas of interest and expertise include cellular mechanisms involved in inflammation and infectious diseases, intracellular transport, cell secretion and advanced electron microscopic techniques.
Dvorak, Ann M.
Weller, Peter F.