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Enzyme Engineering and Evolution, Including Directed Evolution, Vol 643. Methods in Enzymology

  • ID: 5007943
  • Book
  • September 2020
  • Region: Global
  • 412 Pages
  • Elsevier Science and Technology
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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
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1. Site-directed recombination (SDR) in vivo: a fast and reliable tool to unveil beneficial epistasis Miguel Alcalde 2. Creation and application of amine oxidase with expanded substrate specificities from porcine kidney D-amino acid oxidase Yasuhisa Asano 3. Methods to assess correlation networks for engineering transketolase Paul Dalby 4. Exploration of Enzyme Diversity by Integrating Bioinformatics with Microfluidics Jiri Damborsky 5. Engineering lytic polysaccharide monoxygenases (LPMOs) Vincent Eijsink 6. Emulsion-based directed evolution of enzymes in yeast. Andrew D. Ellington 7. Generation of myoglobin-based carbene transferases with a non-native cofactor Rudi Fasan 8. Loop remodeling for generating the novel function of enzymes Yan Feng 9. Rieske non-heme iron dioxygenases Bernard Hauer 10. Biocatalytic synthesis of enantioenriched aldol adducts using artificial carboligases Donald Hilvert 11. Choosing parameters for the consensus sequence approach for stabilization of proteins Romas Kazlauskas 12. Structure-guided engineering of the substrate specificity and catalytic activity of an enzyme Hak-Sung Kim 13. Exploring the substrate space of transglutaminase in the frame of antibody engineering (Glutamine walking.....) Harald Kolmar 14. Biological fitness landscapes by deep mutational scanning Marc Ostermeier 15. Glutamine-walking: creating reactive substrates for transglutaminase-mediated protein labelling Joelle Pelletier 16. Focused Rational Site-specific Iterative Saturation Mutagenesis (FRISM) Qi Wu 17. Statistical epistasis analysis to elude mutational networks in the evolution of new enzyme functions Nobuhiko Tokuriki 18. Directed evolution of stereoselective aldolases Mikael Widersten 19. Ultra-highthroughput screening for novel protease specificities Sven Panke 20. Directed evolution of transcriptional switches using dual selector systems Daisuke Umeno 21. Circular permutation: a different way to engineer enzyme structure and function Stefan Lutz 22. Methods for directed evolution and evaluation of DNA ligases David F. Ackerley 23. Polymerase Evolution by Droplet-base Optical Polymerase Sorting (DrOPS) John Chaput 24. The use of consensus sequence information to engineer stability and activity in proteins Doug Barrick 25. Machine-learning-guided protein engineering Mehdi D. Davari 26. Generation of myoglobin-based carbene transferases with a non-native cofactor Rudi Fasan 27. Development of a Mass Spectrometry based High Throughput Screening Method for Directed Evolution of Enzymes Huimin Zhao 28. Split enzymes: Design principles and strategy James A. Wells 29. Ultrahigh throughput screening for protein function in droplets Florian Hollfelder
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