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Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry

  • ID: 5029549
  • Book
  • November 2020
  • Region: Global
  • 300 Pages
  • Elsevier Science and Technology
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Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry introduces scientists to IMS, describing its many applications for diverse classes of biomolecules. The book is divided into two sections, with the first building a foundation of IMS knowledge by introducing sample preparation, visualization guidelines, molecule identification, quantification, and software and hardware tools. The second section contains chapters focused on each biomolecule class (sugars, polar metabolites, and so on). All chapters include an introduction/background, review of complementary non-IMS techniques, a summary of successful IMS case studies with illustrative figures, a table (showing tissue, condition, ionization modality, and more), and future directions.

The second part of the book can be used by both novices and experts looking for a quick deep-dive into IMS for a specific class of molecule used in their research.

  • Provides the introductory foundations of Imaging Mass Spectrometry for those new to the technique
  • Organized by molecule class to facilitate a quick, deep-dive, allowing researchers to immediately apply these techniques to their work
  • Includes a table in every molecule class chapter, summarizing the IMS experiments done for each class of molecules
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Section I: Imaging Mass Spectrometry Basics 1. Introduction to imaging mass spectrometry 2. Sample preparation guidelines 3. Molecule identification strategies 4. Quantitation workflow in imaging mass spectrometry 5. Molecule visualization schemes (normalization, scale, color schemes) 6. Software 7. Hardware

Section II: Biomolecule Mapping by Imaging Mass Spectrometry 8. Sugars 9. N-Glycan 10. Gangliosides 11. Amino Acids 12. Neuropeptides 13. Ceramides 14. Sphingomyelins 15. Phospholipids 16. Secondary plant metabolites 17. TCA cycle metabolites 18. Neurotransmitters 19. Drugs
examples 20.Steroids 21. ADP, ATP 22. Bacterial metabolites 23. Fatty acids 24. Triglyceride 25. Proteins

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Shrestha, Bindesh
Dr. Bindesh Shrestha is a senior scientist at the Waters Corporation focusing on imaging mass spectrometry. He has co-authored more than two dozen peer-reviewed articles in the field of mass spectrometry spanning over the last decade. His publications have been featured multiple times on the covers of leading journals, including Angewandte Chemie, Analytical Chemistry, Analyst, and more.
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