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Linkers in Biomacromolecules, Vol 647. Methods in Enzymology

  • ID: 5130656
  • Book
  • January 2021
  • 412 Pages
  • Elsevier Science and Technology
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Linkers in Biomacromolecules, Volume 647 in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. New sections in this updated release include chapters devoted to ser-gly linkers + models, sergGly- EAAAK linkers, Effect of crowding on linker behavior, Protathether. Experimental approach to quickly test different linkers in fusion proteins, Linkers in enzyme fusions/cascade catalysis, Linker dependence of avidity in multivalent interactions between disordered proteins, ER/K ?-helix linker, The effect of chirality and steric hindrance on intrinsic backbone conformational propensities: tools for protein design, Linker design in protein-based nanostructures, and much more. Linkers in Biomacromolecules, Volume 647 in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. New sections in this updated release include chapters devoted to ser-gly linkers + models, sergGly- EAAAK linkers, Effect of crowding on linker behavior, Protathether. Experimental approach to quickly test different linkers in fusion proteins, Linkers in enzyme fusions/cascade catalysis, Linker dependence of avidity in multivalent interactions between disordered proteins, ER/K ?-helix linker, The effect of chirality and steric hindrance on intrinsic backbone conformational propensities: tools for protein design, Linker design in protein-based nanostructures, and much more.
  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
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1. ser-gly linkers + models Maarten Merkx 2. sergGly- EAAAK linkers Xin-Hui Xing 3. Effect of crowding on linker behaviour Arnold Boersma 4. Protathether. Experimental approach to quicly test different linkers in fusion proteins Robert Murray Hughes IV 5. Linkers in enzyme fusions/cascade catalysis Marco Fraaije 6. Linker dependence of avidity in multivalent interactions between disordered proteins Magnus Kjaergaard 7. ER/K ?-helix linker Sivaraj Sivaramakrishnan 8. The effect of chirality and steric hindrance on intrinsic backbone conformational propensities: tools for protein desig Valerie Daggett 9. Linker design in protein-based nanostructures; EAAAK linkers Ryoichi Arai 10. Synthetic Protein Switches: Combinatorial Linker Engineering with iFLinkC Viktor Stein 11. Oligonucleotide linkers for mechanical control of protein activity Giovanni Zocchi 12. SpyTag/SpyCatcher as linker to create alternative protein topologies Fei Sun
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