Linkers in Biomacromolecules, Volume 647 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New sections in this updated release include chapters devoted to ser-gly linkers + models, sergGly- EAAAK linkers, Effect of crowding on linker behavior, Protathether. Experimental approach to quickly test different linkers in fusion proteins, Linkers in enzyme fusions/cascade catalysis, Linker dependence of avidity in multivalent interactions between disordered proteins, ER/K a-helix linker, The effect of chirality and steric hindrance on intrinsic backbone conformational propensities: tools for protein design, Linker design in protein-based nanostructures, and much more. Linkers in Biomacromolecules, Volume 647 in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. New sections in this updated release include chapters devoted to ser-gly linkers + models, sergGly- EAAAK linkers, Effect of crowding on linker behavior, Protathether. Experimental approach to quickly test different linkers in fusion proteins, Linkers in enzyme fusions/cascade catalysis, Linker dependence of avidity in multivalent interactions between disordered proteins, ER/K a-helix linker, The effect of chirality and steric hindrance on intrinsic backbone conformational propensities: tools for protein design, Linker design in protein-based nanostructures, and much more.
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Table of Contents
Preface Maarten Merkx
1.Understanding and applications of Ser/Gly linkers in protein engineering Eric Ceballos-Alcantarilla and Maarten Merkx
2.Design and construction of chimeric linker linkers with controllable flexibilities for precision protein engineering Ziliang Huang, Chong Zhang and Xin-Hui Xing
3.Engineering crowding sensitivity into protein linkers Theodoros Pittas, Weiyan Zuo and Arnold J. Boersma
4.protaTETHER: A method for the incorporation of linkers in biomacromolecules Anna I. Wurz, Collin T. O'Bryant and Robert M. Hughes
5.Optimizing the linker length for fusing an alcohol dehydrogenase with a cyclohexanone monooxygenase Alejandro Gran-Scheuch, Friso Aalbers, Yannick Woudstra, Loreto Parra and Marco W. Fraaije
6.Predicting the effect of disordered linkers on effective concentrations and avidity with the "Ceff calculator� app Magnus Kjaergaard, Juliana Glavina and Lucia Beatriz Chemes
7.ER/K-link-Leveraging a native protein linker to probe dynamic cellular interactions Tejas M. Gupte, Michael Ritt and Sivaraj Sivaramakrishnan
8.Design of helical linkers for fusion proteins and protein-based nanostructures Ryoichi Arai
9.Synthetic protein switches: Combinatorial linker engineering with iFLinkC Alexander Gr�we, Jan Ranglack, Anastasia Weyrich and Viktor Stein
10.Enzyme-DNA chimeras: Construction, allostery, applications Chiao-Yu Tseng, Yong Wang and Giovanni Zocchi
11.The Spy that links: Creation of nonlinear protein architectures and materials using SpyTag/SpyCatcher chemistry Ri Wang and Fei Sun
