Topics covered in part 2 include:
- Fourier transform mid-infrared (FT-MIR) spectroscopy
- High performance liquid chromatography (HPLC)
- Raman spectroscopy
- Circular dichroism (CD) spectroscopy
- Transmission electron microscopy (TEM)
- Scanning electron microscopy (SEM)
- SEM-EDX and its applications in plant science
This book is a simple, useful handbook for students and teachers involved in graduate courses in life sciences and medicine. Readers will learn about the basics of featured techniques, the relevant applications and the established protocols.
Table of Contents
Chapter 1 Fourier-Transform Mid-Infrared (Ft-Mir) Spectroscopy In Biomedicine
- Consent For Publication
- Conflict Of Interest
- Acknowledgements
- References
Chapter 2 High Performance Liquid Chromatography (HPLC) Theoretical And Practical Aspects
1. Introduction
1.1. Dispersive Versus Interferogram Based Equipment
1.2. Types Of Samples, Detection Modes And Data Analysis Methods
1.3. Ft-Mir Spectroscopy And Systems Biology
1.4. Nir Spectroscopy (Nirs)
1.5. Raman Spectroscopy
2. Data Acquisition Modes, Pre-Processing And Processing Methods 12
2.1. Spectra Acquisition Modes
2.2. Quality Tests And Spectral Pre-Processing
2.3. Biochemical And Metabolic Interpretation
2.4. Dimensionality Reduction
2.5. Pattern Recognition And Multivariate Regression
2.6. Advanced Algorithms
3. Applications In Clinical Diagnosis
3.1. Cancer
3.2. Brain Disorders
4. Final Remarks
4.2. Quantitative Analysis
5. HPLC Applications
- Consent For Publication
- Conflict Of Interest
- Acknowledgements
- References
Chapter 3 Raman Spectroscopy And Its Biomedical Applications
1. Introduction
2. Theoretical Background
2.1. Raman Scattering
2.2. Instrumentation For Raman Spectroscopy
2.3. Instrument Set Up
2.3.1. Source
2.3.2. Sample Handing Techniques
2.3.3. Filter
2.3.4. Monochromator
2.3.5. Detector
2.4. Scanning Of Raman Spectra
3. Techniques Of Raman Spectroscopy
3.1. Confocal Raman Spectroscopy
3.2. Raman Micro Spectroscopy
3.3. Selective-Sampling Raman Micro-Spectroscopy
3.4. Surface Enhanced Raman Spectroscopy
3.5. Spatially Offset Raman Spectroscopy And Transmission Raman Spectroscopy
3.6. Fiber-Optic Raman Probes
3.7. Coherent Anti-Stokes Raman Spectroscopy And Stimulated Raman Spectroscopy
4. Biomedical Applications
4.1. Raman Based Study Of Human Skin
4.2. Measurement Condition
4.3. In Vivo Cancer Detection By Raman Spectroscopy
4.4. Biofluid Diagnostic Assays Based On Raman Spectroscopy
4.5. Raman Analysis Of Sperm Nuclear Dna Integrity
4.6. Raman Investigation Of Microorganisms On Single Cell Level
4.7. Investigating The Atherosclerosis Process
4.8. Thrombosis Mechanisms Using High Resolution Surface Enhanced Raman Spectroscopy
4.9. Structure Determination Of Gelonin As A Biomolecule By Raman Spectroscopy
4.9.1. Classification
4.9.2. Measurement
4.9.3. Assignment Of Proteins
- Conclusion
- Consent For Publication
- Conflict Of Interest
- Acknowledgements
- References
Chapter 4 Circular Dichroism
1. Introduction
2. Instrumentation And Sample Preparation
3. Data Collection And Processing
4. Applications
4.1. Secondary Structure Content Of Proteins
4.1.1. Detecting Conformational Alterations Upon Ligand Binding
4.2. Protein Stability
5. Protocol For Cd Measurement
6. Summary
- Consent For Publication
- Conflict Of Interest
- Acknowledgements
- References
Chapter 5 Transmission Electron Microscopy: Theory And Applications
1. Introduction
2. Theory
3. Instrumentation
4. Preparation Of Samples And Working Applications
5. Challenges With Biological Electron Microscopy
5.1. Vacuum
5.2. Lack Of Contrast
5.3. Transparency
5.4. Charging
6. Development Of Scanning Transmission Electron Microscope
- Conclusion
- Consent For Publication
- Conflict Of Interest
- Acknowledgements
- References
Chapter 6 Scanning Electron Microscopy: Theory And Applications
1. Introduction
2. Instrumentation
3. Sample Preparation
4. Applications Of Scanning Electron Microscopy (Sem)
- Consent For Publication
- Conflict Of Interest
- Acknowledgements
- References
Chapter 7 Sem-Edx: A Potential Tool For Studies Of Medicinal Plants
1. Introduction
2. Details Of Sem-Eds
3. Principle, Working And Procedure
4. Literature Review With Emphasis On Sem-Edx Study Of Medicinal Plants
5. Medicinal Plants In Jharkhand State: An Overview
6. Significance
- Conclusion
- Consent For Publication
- Conflict Of Interest
- Acknowledgements
- References
- Subject Index
Author
- Yusuf Tutar
