Pore Forming Toxins, Volume 649 in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by field leaders. Chapters in this new release include X-ray crystallography shines a light on pore-forming toxins, Giant unilamellar vesicles for studying activity of pore forming proteins, Combined applications of fluorescence spectroscopy and molecular dynamics simulations in studies of Diphtheria Toxin translocation domain, Biophysical approaches to study actinoporin-lipid interactions, Molecular basis for activation of Actinoporins by lipids, Engineered ClyA for detection of biological molecules, Pore-forming Toxins for the Size-Discrimination of Polymers and Biopolymers: Towards Biomolecules Sequencing, and much more.
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Table of Contents
1. X-ray crystallography shines a light on pore-forming toxins Michael Parker 2. Giant unilamellar vesicles for studying activity of pore forming proteins Gregor Anderluh 3. Combined applications of fluorescence spectroscopy and molecular dynamics simulations in studies of Diphteria Toxin translocation domain Alexey S. Ladokhin 4. Biophysical approaches to study actinoporin-lipid interactions Sara Garcia-Linares 5. Molecular basis for activation of Actinoporins by lipids Kouei Tsumoto 6. Engineered ClyA for detection of biological molecules Giovanni Maglia 7. Pore-forming Toxins for the Size-Discrimination of Polymers and Biopolymers: Towards Biomolecules Sequencing Abdelghani Oukhaled 8. Use of Pore-Forming Toxins to study co-translocational protein folding David Rodriguez-Larrea 9. The use of pore-forming toxins to image lipids and lipid domains Toshihide Kobayashi 10. Engineering outer membrane porin for protein detection Min Chen 11. The use of Ostreolysin A and Anthrolysin O to study cholesterol on cell membranes Arun Radhakrishnan 12. Studying the mechanism of Pore-forming Toxins using High-speed Atomic Force Microscopy Simon Scheuring 13. The use of Atomic-Force Microscopy to study Pore-Forming Toxins Bart Hoogenboom 14. Cysteine-based crosslinking approach for characterization of oligomeric pore-forming proteins in the mitochondrial membrane Jialing Lin 15. High-resolution structure and the sample preparation of Clostridium perfringens iota-toxin pore complex Hideaki Tsuge 16. How protein engineering has revealed the molecular mechanisms of pore-forming toxins? Rodney Tweten 17. Single Molecule Fluorescence Techniques to Study the Mechanism of Pore-Forming Toxins Mark Wallace 18. Cryo-EM Robert Gilbert 19. Going full circle: determining the structures of complement component Michelle Dunstone 20. Different MD simulation techniques used to study Pore-forming Toxins Ganapathy Ayappa 21. Contribution of Molecular Dynamics Simulations to the study of Pore Forming Toxins Gerhard Hummer 22. Membranes with lipid asymmetry for studying Pore-Forming Toxins Erwin London 23. Topological analysis of Pore-forming proteins in native membranes Alejandro Heuck
