Methods in Enzymology, Volume 650 continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Biophysical methods to study lanthanide-protein interactions, Genetically encoded sensors to study lanthanide biology, Spectrophotometric methods to determine the stability constants of lanthanide-macromolecule complexes, Lanthanide-based probes for amino acid modifications, In vitro selection and application of lanthanide-dependent DNAzymes, LRET biosensors for imaging protein interactions in living cells, Synthetic Modeling of the Structure and Function of the Lanthanide-Dependent, MDH Cofactor, EPR spectroscopy of lanthanides, Macromolecular crystallography for f-element complex characterization, and much more.
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Table of ContentsDiscovery of lanthanide-dependent methylotrophy and screening methods for lanthanide-dependent methylotrophs
Synthetic Modeling of the Structure and Function of the Lanthanide-Dependent, MDH Cofactor
Activity assays of methanol dehydrogenases
Expression, purification and properties of enzymes involved in lanthanide-dependent alcohol oxidation: XoxF4, XoxF5, ExaF/PedH, XoxG4
Expression, purification and testing of lanthanide-dependent enzymes in Methylorubrum extorquens AM1
Norma Cecilia Martinez-Gomez
Heterologous expression, purification, and characterization of proteins involved in lanthanide utilization
Joseph A. Cotruvo Jr.
Transposon mutagenesis for methylotrophic bacteria using Methylorubrum extorquens AM1 as a model system
Metals and Methylotrophy: Global Gene Expression Studies
Marina G. Kalyuzhnaya
Mass spectrometry-based approaches to study lanthanides in the phyllosphere
Julia A. Vorholt
Cultivation techniques to study lanthanide metal interactions in the haloalkaliphilic Type I methanotroph "Methylotuvimicrobium buryatense" 5GB1C
Joseph Douglas Groom Jr.