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Recombinant Protein Expression Part A. Methods in Enzymology Volume 659

  • ID: 5342466
  • Book
  • November 2021
  • Elsevier Science and Technology

Recombinant Protein Expression, Part A, Volume 659 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more.

Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr® 250,  and Expression of recombinant antibodies in Leishmania tarentolae.

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Preface Zvi Kelman and William Brad O'Dell 1. Multiplexed analysis protein:protein interactions of polypeptides translated in Leishmania cell-free system Kirill Alexandrov 2. MultiBac system and its applications, performance and recent Imre Berger 3. Production of antibodies in Shuffle Mehmet Berkmen 4. Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast PINAR CALIK 5. Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast PINAR CALIK 6. Antibody or protein-based vaccine production in plants QIANG Chen 7. Cell-free protein synthesis Shaorong Chong 8. Plant-based expression of biologic drugs Keith Davis 9. Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins Venkat Gopalan 10. Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry Venkat Gopalan 11. Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli Sarah W. Harcum 12. Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr® 250 Sarah W. Harcum 13. Expression of recombinant antibodies in Leishmania tarentolae Zoltan Konthur 14. Expression of recombinant antibodies in Halomonas elongate Hans-Jörg Kunte 15. Impact of HDAC inhibitors on recombinant antibody glycosylation Kelvin H. Lee 16. Glutamine auxotrophy in HEK293 cells by means of CRIPSPR gene depletion as a tool for recombinant protein expression Martí Lecina i Veciana 17. Overexpression and purification of secreted 'salt-loving' proteins in Haloferax volcanii: a case study of laccase Julie A. Maupin-Furlow 18. Overexpression and purification of cytoplasmic 'salt-loving' proteins in Haloferax volcanii: a case study of 20S proteasomes Julie A. Maupin-Furlow 19. Transient expression of recombinant proteins in plants Kenji Miura and Shohei Nosaki 20. Controlled deuterium incorporation using fed-batch fermentation approaches Hugh O'Neill 21. Biotechnology applications of cell-free expression systems Javin P. Oza 22. Recombinant production of membrane proteins in yeast Per Amstrup Pedersen 23. Transient expression of recombinant proteins in plants using Potato Virus X based vectors Nikolai V. Ravin 24. Chaperone enhanced expression Frank Robb 25. From the notebook to recombinant protein production in Escherichia coli: design of expression vectors and gene cloning Germán L. Rosano 26. Starting a new recombinant protein production project in Escherichia coli Germán L. Rosano 27. Guidelines for nucleic acid template design for optimal cell-free protein synthesis using an E. coli reconstituted system or a lysate-based system James C. Samuelson 28. Recombinant protein expression in Sulfolobus islandicus Qunxin She and Xu Feng 29. Purification of ribonucleoprotein effector complexes of CRISPR-Cas systems from Sulfolobus islandicus Qunxin She and Mingxia Feng 30. Recombinant protein expression in yeast Mike O'Donnell 31. Expression of proteins in Pichia pastoris Karen Marie Polizzi 32. Cell-free protein synthesis using Chinese Hamster Ovary cells Karen Marie Polizzi 33. Exploring the boundary conditions of AAV Transient Transfection in Suspension Cell Lines John Kerwin 34. Fusing an insoluble protein to GroEL apical domain enhances soluble expression in Escherichia coli Prasad Reddy 35. Thermococcus kodakarensis provides a versatile hyperthermophilic archaeal platform for protein expression Thomas James Santangelo 36. A fast-track protocol for protein expression using the BEV system Sabine Suppmann 37. Protein expression in piggybac transposase mediated stable HEK293 cell pools Sabine Suppmann 38. Applications of Golden Gate cloning for the baculovirus expression vector system Peggy Stolt-Bergner
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