Planar Gel Electrophoresis: For Protein Separation presents the state-of-the-art in planar gel electrophoretic techniques and how they can be implemented in laboratory practice for protein separation. The book covers the whole of the subject, including its historical background, concepts, applications and new developments in planar gel electrophoresis in protein separation. Chapters are written by experts with deep experience in their field. This volume serves as a practical guide for researchers to choose and utilize suitable sample preparation procedures, also describing notable contemporary uses in planar gel electrophoresis in industrial and academic project environments.
Table of Contents
Section 1: History, Theory and Milestones 1. Historical backgrounds and milestones 2. Theory and core concepts 3. Different gel chemistry and buffer systems
Section 2: Sample preparation 4. Preparation of protein samples
Section 3: Different electrophoretic techniques 5. SDS-PAGE (Denaturing gels) 6. Non-denaturing gels 7. Non-reducing gels 8. Gradient gels to cover high MW and low MW proteins 9. Separation of low MW peptides with Tris-Tricine gels 10. Agarose Gels for Separating High MW Proteins 11. Preparative gels 12. Acid- urea triton gel for separating baisc proteins and histones 13. Blue native gels for separating protein complex 14. Pore gradient gel electrophoresis (PGGE) 15. Cationic Electrophoresis 16. Isoelectric focusing (IEF) 17. Immobilized pH gradients (IPG) 18. Two-dimenstional gel electrophoresis (2-DE) 19. Difference gel electrophoresis (DIGE) 20. Diagonal Electrophoresis for disulfide bond detection 21. Affinity gel electrophoresis (AEP) 22. Electrophoretic mobility shift assays (EMSA) 23. Immobilized metal affinity electrophoresis (IMAEP) 24. 1D and 2D zymography (1DZ and 2DZ) 25. SARCOSYL-PAGE: separation of PEGylated proteins. 26. Phenylboronate acrylamide gel electrophoresis for glycated proteins 27. Field Inversion gel electrophoresis (FIGE) 28. Microfluidic gel electrophoresis
Section 4: Post-electrophoresis analysis 29. Visualization of gel separated proteins with protein stains 30. Densitometry analysis of protein bands or spots 31. Data analysis software for extracting information from gel separations 32. Recovery of proteins from gel with electroelution 33. In-gel proteolytic digestion of proteins and mass spectrometry
Section 5: Coulping electrophoresis with antibody detection 34. Western Blotting 35. Serological Proteome Analysis (SERPA) 36. Immunofixation Electrophoresis (IFE) 37. Immunoelectrophoresis (IEP)
