Principles and Practice of Ovarian Tissue Cryopreservation and Transplantation provides methods and techniques of ovarian tissue harvesting and cryopreservation, including instructional videos. This book will benefit a wide audience, guiding infertility specialists, fellows, residents, reproductive surgeons, reproductive endocrinologists, pediatric surgeons, embryologists, infertility nurses and gynecologists. Ovarian cryopreservation and transplantation is rapidly gaining acceptance as a successful and established fertility preservation strategy in cancer patients and beyond. Unlike other fertility preservation strategies, it can be performed in children as well as adults, and can be helpful in restoring natural ovarian function and fertility, hence this is a welcomed resource on the topic.
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Table of Contents
1. Scientific History of Ovarian Transplantation with Cryopreserved Tissue 2. Animal Models of Ovarian Transplantation 3. Overall Approach to Fertility Preservation and Indications for Ovarian Tissue Harvesting and Cryopreservation 4. Regulatory Aspects of Ovarian Tissue Cryopreservation and Transplantation: How to Set Up a Program in Your Center 5. Ovarian Reserve and Early Follicle Development: Prerequisite Knowledge for Understanding Ovarian Tissue Harvesting for Cryopreservation 6. The Methods and Techniques of Ovarian Tissue Harvesting and Preparation before Cryopreservation 7. Oocyte Retrieval and In Vitro Maturation from the Harvested Tissue Before Cryopreservation 8. A Primer on Cryobiology and Cryoprotectants for Ovarian Tissue Freezing 9. Methods of Ovarian Tissue Cryopreservation: Slow Freezing 10. Methods of Ovarian Tissue Cryopreservation: Vitrification 11. Pre-op Evaluation and Preparation for Ovarian Tissue Transplant Surgery 12. Assessment of the Risk of Cancer Cell Reintroduction Prior to Ovarian Tissue Auto-transplantation 13. Ovarian Cryopreservation for Transplantation with Prepubertal and Adolescent girls 14. Surgical Approach to Orthotopic Ovarian Tissue Transplantation 15. Surgical Approach to Laparoscopic and Robot-Assisted Ovarian Tissue Transplantation 16. Surgical Approach to Heterotopic Ovarian Tissue Transplantation Techniques 17. Postovarian Transplantation Follow Up 18. Oocyte Retrieval and IVF Approaches in Patients with Transplanted Ovarian Tissue 19. Ovarian Tissue Cryopreservation for Delaying Childbearing and Menopause 20. In Vitro Growth and Maturation of Primordial Follicle Oocytes from Cryopreserved Tissue 21. Psychosocial and ethical aspects of ovarian tissue cryopreservation in children and adults 22. Future Aspects of Ovarian Cryopreservation and Transplantation 23. Appendix 1. Links to Instructional Videos 24. Appendix 2. Materials, Equipment and Protocols for Ovarian Tissue Cryopreservation and Thawing 25. Appendix 3. Materials, Equipment and Operating Room Set-Up for Ovarian Tissue Transplantation
