Methods in Enzymology serial highlights new advances in the field with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
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Table of Contents
1. Fluorescence loading assay for hexameric helicases James Berger 2. Use of substrates with multiple fluorophores to simultaneously monitor unwinding of multiple duplexes Alicia Byrd 3. In silico reconstitution of MCM helicase loading and activation using time resolved cryo-EM Alessandro Costa 4. Helicase uncoupling to study nacent strand decay James Dewar 5. Investigating structure and dynamics of Twinkle helicase with Cryo-EM and high-speed AFM Yang Gao 6. Measuring helicase contributions to BIR Greg Ira 7. Crosslinking to define helicase/DNA interactions James L. Keck 8. Use of Reverse Polarity DNA to investigate Helicase Mechanisms Tim Lohman 9. Substrate production to study helicases and translocases using molecular and optical tweezers Fernando Moreno Herrero 10. Methods of loading CMG onto DNA Michael O'Donnell 11. Methods to study Helicase-Polymerase coupling during DNA replication reactions Smita Patel 12. Biochemical analyses of ATP-dependent chromatin remodeling Craig Peterson 13. Alignment of helicases on single-stranded increases activity Kevin D. Raney 14. CMG helicase activity on G4-containing templates Dirk Remus 15. Accessory helicase unwinding of genomic complexities Grant Schauer 16. Monitoring helicase decoupling in bacteria by TUNEL Michael Trakselis 17. the preparation of DNA constructs for the use in single-molecule replisome studies Antoine Van Oijen 18. Interaction of topoisomerases with DNA measured by RADAR Alessandro Vindigni