Cryo-electron Tomography: A Journey from Sample Preparation to Data Mining offers a comprehensive guide to the latest state-of-the-art methods and techniques available to researchers. The book begins with a section on sample preparation, considering a range of different sample types including virus-like particles, viruses, bacteria, organoids and tissues, with some chapters dedicated to specific tools like in situ cellular tomography and correlative techniques. The book then moves on to a section on data mining and specific steps and methods, delivering a complete overview of this advancing technique and providing researchers with the knowledge and tools required for their own investigations.
Table of Contents
Part I: Sample Preparation 1. VLP (Virus-like particles) 2. Viruses 3. Bacteria 4. n situ cellular tomography 5. Correlative techniques 6. Host pathogen interactions 7. Towards organoids and tissues Part II: Data Mining 1. Sub-tomogram averaging 2. Segmentation 3. Denoising 4. Feature detection 5. Validation
Authors
Dorit Hanein Department of Structural Biology and Chemistry, Institut Pasteur; and Department of Chemistry and Biochemistry, Scintillon Institute San Diego, USA. Professor Hanein has over 25 years of experience in developing and applying cryo-EM technology for high-resolution structural studies of biological macromolecules and cells. Her research is at the interfaces between structural biology, cell biology, systems biology, and engineering science. Prof. Hanein is leading efforts in combining light microscopy with cellular tomography to permit the placement of dynamic multimolecular protein complexes into their functional context in whole mammalian cells. Through these efforts, she made seminal contributions to our knowledge of the structure, assembly and regulation of actin cytoskeleton and its associated macromolecular assemblies, to the development of techniques and protocols for correlative light and in-situ cellular tomography, and to the evolution of quantitative cryo-EM. Niels Volkmann Bioinformatics and Structural Biology Program, Sanford-Burnham Medical Research Institute, California; Structural Image Analysis Unit, Department of Structural Biology and Chemistry, Institut Pasteur, USA. Niels Volkmann earned his Ph.D. in Biophysics from the University of Hamburg, Germany in 1993, where he also trained as a postdoctoral fellow at the Max Planck Research Unit for Ribosome Structure. He received additional postdoctoral training at Brandeis University at the Rosenstiel Basic Medical Science Research Center and The Keck Center for Cellular Visualization prior to joining Sanford Burnham Prebys in 1999. Dr. Volkmann was promoted to the faculty as Assistant Professor in 2001.Dr. Volkmann's lab seeks to understand biological processes as integrated, hierarchical systems rather than as isolated parts, by developing and applying quantitative analysis tools to link and integrate the scales of atomic-resolution information and imaging of dynamic events in living cells. A key feature of Dr. Volkmann's research is the highly synergistic collaborations between his laboratory and other research groups (particularly Dr. Hanein's) that are addressing fundamental questions in cell and cancer biology.
