Correlative Light and Electron Microscopy V. Methods in Cell Biology Volume 187

Table of Contents

1. How to Apply the Broad Toolbox of Correlative Light and Electron Microscopy to Address a Specific Biological Question
Thomas M�ller-Reichert, Erin M. Tranfield, Gunar Fabig and Thomas Kurth
2. Some tips and tricks for a correlative light electron microscopy workflow using stable expression of fluorescent proteins
Paul Verkade
3. Targeting membrane receptors with fluoronanogold probes for high resolution correlative microscopy.
Monica Fernandez Monreal
4. Correlative light and electron microscopy at defined cell cycle stages in a controlled environment
Shotaro Otsuka
5. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) culture and sample preparation for correlative light electron microscopy
Paul Verkade, Maximillian Erdmann, Isobel Web, Lorna Hodgson and Andrew Davidson
6. Tissue CLEM
Christine Longin
7. Array tomography of in vivo labeled synaptic receptors
Christian Stigloher and Sebastian Britz
8. Correlative cryo-microscopy pipelines for in situ cellular studies
Anna Sartori and Chiara Zurzolo
9. Building a super-resolution fluorescence cryomicroscope
Thom Sharp
10. Analysis of super resolution CLEM data
Thom Sharp
11. Nanometer targeting using cryo super resolution
Laura Carolina Zanetti-Domingues
12. Laboratory Based Correlative Cryo-Soft X-Ray Tomography and Cryo-Fluorescence Microscopy
Kenneth Fahy

Authors

Thomas M�ller-Reichert Core Facility Cellular Imaging (CFCI), Faculty of Medicine Carl Gustav Carus, Technische Universit�t Dresden, Germany. Thomas M�ller-Reichert works in the Core Facility Cellular Imaging (CFCI), Faculty of Medicine Carl Gustav Carus, Technische Universit�t Dresden, Germany. Paul Verkade The University of Bristol, Bristol, UK. Paul Verkade is a Professor of Bioimaging at the University of Bristol, UK where his research group works on the development and application of microscopy techniques to Biomedical questions. The main tools in the lab are Electron microscopy (EM) and Correlative Light Electron Microscopy (CLEM) in which fields he has published over 100 papers and edited 5 books on CLEM (including 4 Volumes of the Methods in Cell Biology series). PV obtained his PhD at the University of Utrecht, The Netherlands in 1996. Subsequently he did a post-doc at the EMBL, Heidelberg, Germany, after which he set up the electron microscopy unit at the newly formed Max Planck Institute for Molecular Cell Biology in Dresden, Germany from 2001. He moved to the UK in 2006 to set up another EM unit as part of an integrated LM and EM bioimaging facility, which facilitates CLEM workflows. He is actively involved in shaping the future microscopy landscape with roles in the Royal Microscopical Society and BioimagingUK and a current focus on putting volumeEM on the imaging map through community building and the organisation and co-chairing of the 1st Gordon Research Conference on vEM.