RNases in Health and Diseases: From Housekeeping Enzymes to Moonlight Proteins provides coverage of the structural and functional properties of RNases, introducing new concepts such as their recently discovered multifaceted properties, their involvement in the generation of non-coding signaling RNAs, and their proposed uses as diagnostic and therapeutic tools. This book's sections cover the structure of RNases, present research methods and tools to study RNases, and delve into RNases and diseases. Users will find a wider perspective on the topics discussed that will serve researchers and students engaged in the study of RNases who are interested in acquiring a better understanding.
Table of Contents
Section I: Structure
1. Overview. RNases, a family of multifaceted proteins
2. Catalytic mechanism of action: mechanism, exonucleases vs endonucleases, single stranded vs double stranded.
3. RNases diversity: vertebrate RNase A superfamily, RNase T2, RNase L, RNase kappa, plant RNase S, bacterial RNases
4. RNase in evolution: RNAs and RNases
5. RNA subsite binding sites
6. From monomers to multimers
7. RNases cellular traffic
8. RNases and tyrosine kinase receptors
9. RNases and posttranscriptional modifications
10. RNases targeting non-coding RNAs
11. RNase Inhibitors
Section II: Research Methods
12. Tools to study RNases:
a) labelled probes
b) HPLC product analysis,
c) Cp-RNAseq…
13. RNase-based tools: RNaseH, RNAse4 for RNA analysis,…CRISPR/Cas9
Section III: Diseases
14. RNases in infectious diseases
15. RNases in inflammatory disorders
16. RNases in cancer
17. Human Natural Variants
18. RNases as diagnostic tools
19. RNases as therapeutic targets
20. RNases and drug design: antitumoral, anti-inflammatory or antimicrobial agents
