Flavoproteins and Flavoenzymes, Volume 738 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including Kirisu2 and KiPaD: global analysis of rapid flavoprotein redox kinetics from stopped-flow multi, ilagros Mendina, A Generic First Approach to Dissecting Flavoenzyme Catalytic Cycles, Removing Covalent Constraints of Protein Loops and Lids by a Split-Gene Strategy, Designing Oxygen Reaction Sites in Flavoprotein Dehydrogenases, Converting Noncovalent Flavoproteins into Covalently Flavinylated Proteins, Complexities of Riboflavin Biosynthesis, Reactions of Riboflavin Catabolism, and much more.
Additional sections cover Ferredoxin-dependent thioredoxin reductase, Thermophilic Methylenetetrahydrofolate Reductases, Ion-Pumping NADH:Quinone Oxidoreductase, Loading E. coli Dihydroorotate Dehydrogenase with Coenzyme Q, and Single-Component Halogenases.
Additional sections cover Ferredoxin-dependent thioredoxin reductase, Thermophilic Methylenetetrahydrofolate Reductases, Ion-Pumping NADH:Quinone Oxidoreductase, Loading E. coli Dihydroorotate Dehydrogenase with Coenzyme Q, and Single-Component Halogenases.
Table of Contents
1. Photo-CIDNP as a Probe of Flavoprotein StructureStefan Weber
2. Kirisu2 and KiPaD: global analysis of rapid flavoprotein redox kinetics from stopped-flow multi-wavelength absorption spectroscopy data
Milagros Mendina
3. A Generic First Approach to Dissecting Flavoenzyme Catalytic Cycles
Bruce Palfey
4. Removing Covalent Constraints of Protein Loops and Lids by a Split-Gene Strategy
Steve Rokita
5. Designing Oxygen Reaction Sites in Flavoprotein Dehydrogenases
Karen Allen
6. Converting Noncovalent Flavoproteins into Covalently Flavinylated Proteins
Marco Fraaije
7. Complexities of Riboflavin Biosynthesis
Audrey L. Lamb
8. Reactions of Riboflavin Catabolism
Tadhg Begley
9. Ferredoxin-dependent thioredoxin reductase
Milagros Mendina
10. Thermophilic Methylenetetrahydrofolate Reductases
Markos Koutmos
11. Ion-Pumping NADH:Quinone Oxidoreductase
Oscar X. Ju�rez
12. Loading E. coli Dihydroorotate Dehydrogenase with Coenzyme Q
Bruce Palfey
13. Single-Component Halogenases
April Lukowski
