Arginase: Structure, Function, and Therapeutic Targeting, Volume 740 in the Methods in Enzymology series, provides a comprehensive, methodology-driven perspective on arginase research spanning molecular, biochemical, and translational domains. Chapters in this new release include Expression, Purification, and Structural Characterization of Human and Leishmania Arginase with Comparative Analysis of Structures from the Protein Data Bank, Synthesis of First-Generation Arginase Inhibitors, Computational and Structure-Guided Design of Arginase Inhibitors, Metal Substitution in Arginase: Experimental Approaches to Study Metal-Dependent Catalysis, Therapeutic Arginase Formulations: PEGylation, Stability, and Advanced Delivery Systems, and much more.
Additional chapters cover Dual ARG1/ARG2 Inhibition: Experimental and Structural Strategies for Inhibitor Design and Optimization, Synthesis of Second-Generation Arginase Inhibitors, Cryo-EM Workflow and Structural Characterization of Arginase-Antibody Complexes, Development and Validation of an Electrochemical Assay for Arginase Activity, Experimental and Structural Analysis of H. pylori Arginase in Gastric Immune Evasion, Agmatinase and Alternative Arginine Utilization Pathways in Bacteria: Biochemical and Functional Perspectives, Catalytic Diversification of the Arginase Fold: Structural and Mechanistic Insights from DcsB, Beyond Arginase: Structural Biology of the Extended Guanidinase Family, Plant Arginase: Structural and Functional Roles in Nitrogen Metabolism, and much more.
Additional chapters cover Dual ARG1/ARG2 Inhibition: Experimental and Structural Strategies for Inhibitor Design and Optimization, Synthesis of Second-Generation Arginase Inhibitors, Cryo-EM Workflow and Structural Characterization of Arginase-Antibody Complexes, Development and Validation of an Electrochemical Assay for Arginase Activity, Experimental and Structural Analysis of H. pylori Arginase in Gastric Immune Evasion, Agmatinase and Alternative Arginine Utilization Pathways in Bacteria: Biochemical and Functional Perspectives, Catalytic Diversification of the Arginase Fold: Structural and Mechanistic Insights from DcsB, Beyond Arginase: Structural Biology of the Extended Guanidinase Family, Plant Arginase: Structural and Functional Roles in Nitrogen Metabolism, and much more.
Table of Contents
Section I: Molecular and Structural Approaches to Arginase Function and Inhibition1. Expression, Purification, and Structural Characterization of Human and Leishmania Arginase with Comparative Analysis of Structures from the Protein Data Bank
E. D'Antonio, Luigi Franklin Di Costanzo and David W. Christianson
2. Synthesis of First-Generation Arginase Inhibitors
F. Sodano
3. Computational and Structure-Guided Design of Arginase Inhibitors
M.A. Fumagalli
4. Metal Substitution in Arginase: Experimental Approaches to Study Metal-Dependent Catalysis
George Georgiou
Section II: Advanced Therapeutic Strategies and Analytical Methods for Arginase
5. Therapeutic Arginase Formulations: PEGylation, Stability, and Advanced Delivery Systems
L. Sloan and M. Rudebeck
6. Dual ARG1/ARG2 Inhibition: Experimental and Structural Strategies for Inhibitor Design and Optimization
M. Grzybowski
7. Synthesis of Second-Generation Arginase Inhibitors
F. Sodano
8. Cryo-EM Workflow and Structural Characterization of Arginase-Antibody Complexes
G. Scapin
9. Development and Validation of an Electrochemical Assay for Arginase Activity
A. Miglione, Luigi Franklin Di Costanzo and Stefano Cinti
Section III: Clinical, Metabolic, and Translational Aspects of Arginase
10. Experimental and Structural Analysis of H. pylori Arginase in Gastric Immune Evasion
A.K. Sau
11. Agmatinase and Alternative Arginine Utilization Pathways in Bacteria: Biochemical and Functional Perspectives
J. French
12. Arginase Deficiency and Urea Cycle Disorders: Clinical and Metabolic Management
N. Brunetti-Pierri
13. Inborn Errors of Metabolism and Arginine Pathways: Experimental and Clinical Approaches
A. Schulze
14. Arginine Metabolism and Protein Balance: Experimental Approaches in Human Physiology
M. P. K. J. Engelen
15. Arginase in Endothelial Dysfunction and Vascular Disease: Experimental and Translational Approaches
W. Caldwell and R. Caldwell
16. Arginase in Cardiovascular and Vascular Inflammatory Disease
W. Durante
Section IV: Evolutionary and Functional Diversity of Arginase and Related Enzymes
17. Catalytic Diversification of the Arginase Fold: Structural and Mechanistic Insights from DcsB
Y. Matoba and Kosuke Oda
18. Beyond Arginase: Structural Biology of the Extended Guanidinase Family
G. Mlynek, S. V. Kundapura and D. Carugo
19. Plant Arginase: Structural and Functional Roles in Nitrogen Metabolism
