Fundamentals of Absorption Spectroscopy offers a clear and comprehensive introduction to the principles, instrumentation, and applications of absorption spectroscopy across UV-Visible, IR, and other spectral regions. Designed for students, researchers, and industry professionals, this book bridges theory and practice with practical illustrations and real-world case studies, providing a solid foundation in both fundamental concepts and contemporary applications in chemistry, biology, medicine, environmental monitoring, and materials science. It fills the gap between overly advanced and overly simplified texts by combining fundamental theory with practical insights, particularly from biological perspectives. This book addresses the need for an updated, student-friendly, and application-oriented resource in absorption spectroscopy. Readers will benefit from structured guidance that connects theoretical principles with experimental data, explains instrumentation and its limitations, and demonstrates applications across diverse fields such as pharmaceuticals, environmental analysis, and biomolecular sciences. The inclusion of case studies and recent advances in spectroscopic technology ensures relevance for both academic study and professional practice.
Table of Contents
Section I. Foundations of Absorption Spectroscopy1. Introduction to Absorption Spectroscopy
2. Theoretical Basis of Absorption Spectroscopy
3. Instrumentation and Experimental Methods of Absorption Spectroscopy
4. Data Analysis and Spectral Interpretation
5. Limitations and Challenges
Section II. Core Absorption Techniques
6. UV-Visible Absorption Spectroscopy
7. Infrared Absorption Spectroscopy (IR/FTIR)
8. Near-Infrared (NIR) Spectroscopy
9. Atomic Absorption Spectroscopy
10. Advanced Absorption Techniques
Section III. Chemistry, Materials, and Environmental Applications
11. Absorption Spectroscopy in Reaction Kinetics and Mechanisms
12. Applications in Materials, Catalysts, and Nanochemistry
13. Solar Energy and Photovoltaics
14. Environmental and Industrial Applications
15. Detection of Trace Elements
16. Photothermal Microscopy. Imaging the Optical Absorption of Single Molecules
Section IV. Biological and Medical Applications
17. Absorption Spectroscopy in Clinical and Pharmaceutical Sciences
18. Spectroscopic Evaluation of Pigments, Vitamins, Additives, Adulterants, Contaminants, and Authenticity Validation of Food Quality and Safety
19. Herbal Medicine and Natural Products
20. Protein Dynamics Studies
21. Nucleic Acids and Biomolecular Studies
22. Protein and Enzyme Studies
23. High-Throughput Steady-State Enzyme Kinetics Measurements through Absorbance Detection
24. Absorption Spectroscopy in Drug Discovery
25. Protein Structure and Folding Pathway Investigation through Absorption Spectroscopy
26. Biosensors and Biomedical Devices
27. Metabolomics, Lipidomics, and Biomarker Detection
28. Applications in Medical and Biological Sciences
29. Prospects of Absorption Spectroscopy for Biomarker Monitoring toward Precision Medicine
Section V. Outlook and Future Directions
30. Modern Data Science in Absorption Spectroscopy
31. Quantitative Analysis Methods for Single-Molecule (smFRET, nanoIR) Experiments
32. Terahertz Absorption Spectroscopy in Biosystems
33. Advanced Techniques and Emerging Methods
34. Future Perspectives and Emerging Trends
