We welcome you to the BioEngineering, BioDetection and BioSensors to be held at the Marriott Coronado Island Resort & Spa, Coronado Island, California on April 1-2, 2019.
BioEngineering 2019 brings together the following topics:
- BioMEMS and Microfluidics-based Devices in Various Application Areas
- Biofabrication Technologies
- Tissue Engineering
- Synthetic Biology
BioDetection and Biosensors 2019 - Rapid growth in the development of biosensors has revolutionized the efficacy and sensitivity of analytical devices across Health, Food and Defenses industries. Yet many challenges remain for such devices and competing forms of assay are still relevant, with some still out performing and providing better value than biosensor-based technologies. Topic and challenges to be discussed include, but are not limited to, Biosensors, Nano Materials and Systems, Next Generation Sequencing for Pathogen Detection, Rapid Biodetection and Threat Identification, Single Molecule Analysis.
There will be excellent opportunities for networking with researchers from academia and industry, providing a platform for sharing insights and addressing challenges that often lead to mutually beneficial collaborations.
Delegates receive full access to the co-located and parallel conference track focusing on BioSensors and BioDetection and the co-located exhibit hall featuring companies displaying the latest technologies, products and services.
*Please Note: Academic pricing provided on enquiry.
Day 1 - BioEngineering Track
Monday, 1 April 2019
- Conference Registration, Materials Pick-Up, Morning Coffee and Pastries
Stephanie Willerth - Keynote Presentation
- 3D Bioprinting Tools For Engineering Complex Neural Tissues From Stem Cells
- Stephanie Willerth, Associate Professor and Canada Research Chair in Biomedical Engineering, University of Victoria, Canada
3D bioprinting can create living human tissues on demand based on specifications contained in a digital file. Such highly customized, physiologically-relevant 3D human tissue models can screen potential drug candidates as an alternative to expensive pre-clinical animal testing. The Willerth lab has developed a novel fibrin-based bioink for bioprinting neural tissues derived from human induced pluripotent stem cells (hiPSCs), which can become any cell type found in the body. Our team uses Aspect Biosystem’s novel RX1 bioprinter featuring Lab-On-a-Printer™ (LOP) technology as it enables us to fabricate complex structures found in healthy neural tissues. The microfluidic LOP™ printhead cartridges generate cell-containing hydrogel fibers of defined diameters that are precisely deposited into defined 3D structures using a sheath fluid that triggers hydrogel cross-linking of the bioink. The sheath fluid also insulates cells within the fiber from shear stress, protecting fragile primary cells from shear-induced cell death. This process allows us to maintain high levels viability (>90% post printing) not previously seen in the literature. Here I will discuss the latest work from our group detailing the composition of our 3D bioprinted tissues and exciting avenues for future work.
Albert Folch - Keynote Presentation
- High-Resolution 3D-Printing of Microfluidics
- Albert Folch, Professor of Bioengineering, University of Washington, United States of America
The vast majority of microfluidic systems are built by replica-molding in elastomers (such as PDMS) or in thermoplastics (such as PMMA or polystyrene). However, biologists and clinicians typically do not have access to microfluidic technology because they do not have the engineering expertise or equipment required to fabricate and/or operate microfluidic devices. Furthermore, the present commercialization path for microfluidic devices is usually restricted to high-volume applications in order to recover the large investment needed to develop the plastic molding processes. Several groups, including ours, have been developing microfluidic devices through stereolithography (SL), a form of 3D printing, in order to make microfluidic technology readily available via the web to biomedical scientists. However, most available SL resins do not have all the favorable physicochemical properties of the above-named plastics (e.g., biocompatibility, transparency, elasticity, and gas permeability), so the performance of SL-printed devices is still inferior to that of equivalent PDMS devices. Inspired by the success of hydrogel PEG-DA biocompatibility, we have developed microfluidic devices by SL in advanced resins that share all the advantageous attributes of PDMS and thermoplastics so that we can 3D-print designs with comparable performance and biocompatibility to those that are presently molded.
Morning Coffee Break and Networking
Maximizing the Impact of Microphysiological Systems with In vitro-In vivo Translation
Murat Cirit, Director at Translational Center of Tissue Chip Technologies, Massachusetts Institute of Technology (MIT), United States of America
A large percentage of drug candidates fail at the clinical trial stage due to a lack of efficacy and unacceptable toxicity, primarily because the in vitro cell culture models and in vivo animal models commonly used in preclinical studies provide limited information about how a drug will affect human physiology. The need for more physiologically relevant in vitro systems for preclinical efficacy and toxicity testing has led to a major effort to develop "Microphysiological Systems (MPS)", aka tissue chips (TC), based on engineered human tissue constructs.
Microphysiological systems hold promise for improving therapeutic drug approval rates by providing more physiological, human-based, in vitro assays for preclinical drug development activities compared to traditional in vitro and animal models. The full impact of MPS technologies will be realized only when robust approaches for in vitro-in vivo (MPS-to-human) translation are developed and utilized, and explain how the burgeoning field of quantitative systems pharmacology (QSP) can fill that need.
- Tissue Manufacturing by Bioprinting: Challenges and Opportunities
- Fabien Guillemot, Chief Executive Officer, Poietis, France
Despite substantial investments to meet clinical and commercial expectations, and while scientific achievements at the preclinical research stage have sometimes been impressive, scaffold-based Tissue Engineering approaches are struggling to find the way to therapeutic and industrial success. Main challenges for the manufacturing of tissue engineered ATMPs concern the improvement of the standardisation of manufacturing processes, tissue functionality, and cost-effectiveness and profitability of related treatments.
Based on our experience in the field of bioprinting, we discuss how this technology - thanks to its characteristics resulting from the convergence of automation, biology and digital technology - should make it possible to overcome current tissue manufacturing bottlenecks and also provide new opportunities.
Michael McAlpine - Keynote Presentation
3D Printed Programmable Release Capsules for Dynamic Tissue Engineering Applications
Michael McAlpine, Benjamin Mayhugh Associate Professor of Mechanical Engineering, University of Minnesota, United States of America
The development of methods for achieving spatiotemporal control over biomolecular gradients could enable advances in areas such as synthetic tissue engineering, biotic-abiotic interfaces, and bionanotechnology. Living organisms guide tissue development through orchestrated gradients of biomolecules that direct cell growth, migration, and differentiation. Our group has previously presented a method to 3D print stimuli-responsive core/shell capsules for programmable release of multiplexed gradients within hydrogel matrices. These capsules are comprised of an aqueous core, which can be formulated to maintain the activity of payload biomolecules, and a PLGA shell. The shell can be loaded with plasmonic gold nanorods (AuNRs), which permits selective rupturing of the capsule when irradiated with a laser wavelength determined by the lengths of the nanorods. This precise control over space, time, and selectivity allows for the ability to pattern 2D and 3D multiplexed arrays of content-loaded capsules, along with tunable laser-triggered rupture and release of payloads into a hydrogel ambient - allowing for dynamic tissue engineering applications. One particular example includes the use of these capsules in the development of 3D in vitro models capable of recapitulating native tumor microenvironments. Here, we build tumor constructs via the co-3D printing of living cells, natural hydrogels, and programmable release capsules. This enables the spatiotemporal control over signaling molecular gradients, thereby dynamically modulating cellular behaviors at the local level. Vascularized tumor models are created to mimic key steps of cancer dissemination (invasion, intravasation, and angiogenesis), based on guided migration of tumor cells and endothelial cells in the context of stromal cells and growth factors. These ‘4D printed’ vascularized tumor tissues provide a proof-of-concept dynamic tissue engineering platform to i) explore the molecular mechanisms of tumor progression and metastasis, and ii) preclinically identify therapeutic agents and screen anticancer drugs.
- Biomimetic Microtopographies Trigger New Potential Therapies For Cornea Replacements
- Christine McBeth, Senior Research Scientist, Fraunhofer Center for Manufacturing Innovation, United States of America
Corneal endothelial cells maintain visual acuity by regulating water content in the cornea. Damage to this region requires corneal transplant from donor tissue. Using 2-photon lithography for molding, a biomimetic membrane triggers pluripotent cells to differentiate into corneal endothelial like cells. This work with Fraunhofer IPT provides foundational understanding of mechanical cues required in this tissue. Our future directions involve scaling this work to translate production to clinical test levels.
- 3D Printing For Cardiovascular Disease Application
- Sara Abdollahi, Researcher, Johns Hopkins University School of Medicine, United States of America
- Narutoshi Hibino, Assistant Professor, Cardiac Surgery, Johns Hopkins Hospital, United States of America
Heart attack can lead to necrosis of myocardial tissue. Stem-cell therapy is an emerging strategy that involves the injection of cardiac progenitor cells to encourage tissue regrowth. However, ongoing trials have showed that the efficacy of this technique is limited and the retention rate of the delivered cells is poor. Cardiac tissue engineering includes the use of cells with biomaterials and signaling molecules to regenerate damaged heart tissue. Herein we present ongoing efforts to optimize cardiac and vascular tissue engineering approaches toward commercial and clinical applications. This includes utilization of additive manufacturing to systematize the process and create patient-matched therapies. These approaches have implications for the treatment of cardiovascular disease and precision medicine.
- Fabrication of Microfluidic Devices with Integrated Electronic Components via Dual Extrusion-Based 3D Printing
- Brandon Strong, Research Assistant, California Polytechnic State University, San Luis Obispo, United States of America
Current microfabrication techniques typically require complex, labor-intensive processes. An alternative method of economical and rapid prototyping is extrusion-based 3D printing. While 3D printing has more recently been applied to the field of microfluidics, channel resolution is poor. Furthermore, since the current generation of microfluidics devices require the integration of electronic components, we utilized dual extrusion-based 3D printing, thereby allowing for the prototyping of multi-material microfluidic devices with integrated electronics. Devices were designed in SolidWorks (modeling software), exported to BCN3D Cura (slicing program), and printed via BCN3D Sigma (dual-extrusion 3D printer). For devices with electronic components, conductive polylactic-acid (PLA) was inlaid within a non-conductive PLA framework to create an internal circuitry.
Afternoon Coffee Break and Networking
- Engineering Human Tissues Using 3D Bioprinting Technology
- Jinah Jang, Assistant Professor, Pohang University of Science And Technology (POSTECH), Korea South
Recent development of bioengineering enables to create human tissues by integrating various native microenvironments, including tissue specific cells, biochemical and biophysical cues. A significant transition of 3D bioprinting technology into the biomedical field helps to improve the function of engineered tissues by recapitulating physiologically relevant geometry, complexity, and vascular network. Bioinks, used as printable biomaterials, facilitate dispensing of cells through a dispenser as well as supports cell viability and function by providing engineered extracellular matrix. Successful construction of functional human tissues requires accurate environments that are able to mimic biochemical and biophysical properties of target tissue. Formulation of printable materials with stem cells are critical process to guide cellular behavior; however, this is rarely considered in the context of bioprinting in which the tissue should be formed. This talk will cover my research interests in building 3D human tissues and organs to understand, diagnose and treat various intractable diseases, particularly for cardiovascular disease. A development of tissue-derived decellularized extracellular matrix bioink platform will be mainly discussed as a straightforward strategy to provide biological and biophysical phenomena into engineered tissues. I will also discuss about a development of 3D vascularized cardiac stem cell patch that is generated by integrating the concept of tissue engineering and the developed platform technologies. Combined with recent advances in human pluripotent stem cell technologies, printed human tissues could serve as an enabling platform for studying complex physiology in tissue and organ contexts of individuals.
- Next-Generation MicroPADs: Merging 3D Printing with Microfluidic Paper-Based Analytical Devices (MicroPADs)
- Brandon Strong, Research Assistant, California Polytechnic State University, San Luis Obispo, United States of America
Microfluidic Paper-Based Analytical Devices (MicroPADs) have emerged as useful diagnostic tools. They possess many key characteristics, including portability, cost-effectiveness, ease of use, low sample volume requirements, and ability to operate without supporting equipment. Researchers have modified microPADs in a myriad of ways to increase functionality, however, the use of 3D printing on microPADs has yet to be explored. The purpose of this project was to use 3D printing to expand microPAD functionality and combat current limitations. MicroPADs were fabricated on chromatography paper via wax printing and affixed to the bedplate of a BCN3D Sigma (dual-extrusion 3D printer). Polylactic acid (PLA) and conductive PLA structures were fabricated on microPADs via 3D printing. 3D fluid reservoirs were fabricated from PLA and allowed for larger sample volumes and further wicking distances on microPADs. Electrodes were fabricated from conductive PLA and allowed for simple electroanalysis on paper. Hybrid devices with both PLA channels (100 µm) and paper channels were also created. PLA-backed hemi- and fully-enclosed paper channels were fabricated, which may allow for increased robustness and reduced contamination of biochemical assays on microPADs, as well as the ability to run devices in direct contact with surfaces (i.e., not held in suspension).
- Microfluidic Multiplexing For Immune Cell Detection In Tumor Sections
- Daniel Migliozzi, Researcher, Ecole Polytechnique Federale de Lausanne, Switzerland
We developed a polymer-and-glass chip for fast delivery of reagents on tissue slides and fully automatic imaging by integration with an optical microscope, along with high-throughput image-processing for single cell mapping. As proof-of-concept analyses, we identified co-expression and co-localization patterns of biomarkers to classify the immune cells and their activation status in lung adenocarcinoma.
Joyce Wong - Keynote Presentation
- Biomaterials for the Early Detection and Treatment of Disease
- Joyce Wong, Professor of Biomedical Engineering and Materials Science & Engineering, Boston University, United States of America
In this talk, I will present two different stories that illustrate the power of biomaterials in clinical applications. The first addresses a major challenge in developing surgical solutions for children with congenital heart disease. Current solutions involve multiple staged surgeries because the implants do not grow with the child. We have developed several methods to generate a layered tissue patch that mimics the cellular organization of native vessels and a bioMEMS device that can be used to assess physiological function of these tissue-engineered constructs with patient data input. The second story addresses our efforts to detect and prevent surgical adhesions, bands of tissue arising from abdominal surgeries that can lead to small bowel obstruction and infertility. The annual cost of adhesion-related complications to the US healthcare system is estimated to be as high as $5 billion. We have developed a novel formulation of ultrasound contrast agents that are stable for up to 4 days and target components found during the initial stage of adhesion formation.
Beer and Wine Reception and Networking
Close of Day 1 of the Conference
3D-Printing in Microfluidics Training Course Presented by Professor Albert Folch, University of Washington
Day 2 - BioDetection & BioSensors Track
Tuesday, 2 April 2019
Morning Coffee, Pastries and Networking
Joseph Wang - Keynote Presentation
- Wearable Electrochemical Sensors: Toward Labs on the Skin or in the Mouth
- Joseph Wang, Chair of Nanoengineering, SAIC Endowed Professor, Director at Center of Wearable Sensors, University of California-San Diego, United States of America
Wearable sensors have received a major recent attention owing to their considerable promise for monitoring the wearer’s health and wellness. The medical interest for wearable systems arises from the need for monitoring patients over long periods of time. These devices have the potential to continuously collect vital health information from a person’s body and provide this information to them or their healthcare provider in a timely fashion. Such sensing platforms provide new avenues to continuously and non-invasively monitor individuals and can thus tender crucial real-time information regarding a wearer’s health. This presentation will discuss recent developments in the field of wearable electrochemical sensors integrated directly on the epidermis or within the mouth for various non-invasive biomedical monitoring applications. Particular attention will be given to non-invasive monitoring of metabolites and electrolytes using flexible amperometric and potentiometric sensors, respectively, along with related materials and integration considerations. The preparation and characterization of such wearable electrochemical sensors will be described, along with their current status and future prospects and challenges.
- Soft Electronics For Noninvasive Healthcare: From the Skin to Below the Skin
- Sheng Xu, Assistant Professor, University of California, San Diego, United States of America
Soft electronic devices that can acquire vital signs from the human body represent an important trend for healthcare. Combined strategies of materials design and advanced microfabrication allow the integration of a variety of components and devices on a stretchable platform, resulting in systems with minimal constraints on the human body. We have demonstrated a skin-mounted multichannel health monitor that can sense local field potentials, temperature, strain, acceleration, and body orientation. Integrating ultrasonic transducers on this stretchable platform adds a third dimension to the detection range by launching ultrasound waves that reach well underneath the skin. The ultrasound waves allow capturing a wide range of dynamic events in deep tissues such as blood pressure and blood flow waveforms in central arteries and veins. This technology holds profound implications for continuous and noninvasive sensing, diagnosis, and treatment of chronic diseases.
- Small Sensors Go Big: Towards High-Resolution Monitoring of Industrial Fermentations
- Helena Junicke, Marie-Curie Researcher, Technical University of Denmark, Denmark
Miniaturized sensors provide new opportunities for industrial fermentation control. In this frequently overlooked application, sensors allow for an improved spatial surveillance of production units, leading to increased performance and lower risk of failure.
Morning Coffee Break
- Phage Display and Directed Molecular Evolution: The Nobel Prize and Beyond. Impact on Material Engineering and Biosensor Development
- Valery Petrenko, Professor, Auburn University, United States of America
Development of phage engineering technology led to construction of a novel type of phage display libraries-collections of nanofiber materials with diverse molecular landscapes accommodated on the surface of phage particles. These new nanomaterials, called "landscape phage," serve as a huge resource of diagnostic/detection probes and versatile construction materials for preparation of phage-functionalized biosensors. Landscape-phage-derived probes interact with biological threat agents and generate detectable signals as a part of robust and inexpensive molecular recognition interfaces introduced in mobile detection devices. The use of landscape-phage-based interfaces may greatly improve the sensitivity, selectivity, robustness, and longevity of these devices. My talk aims to attract the attention of chemical scientists and bioengineers seeking to develop functionalized materials and use them in different areas of bioscience, medicine, and engineering.
- Sensors for Biosensors: Efficient Strategies Towards Successful Screening and Commercialization
- Daria Semenova, Researcher, Technical University of Denmark, Denmark
Novel strategies towards biosensor design optimization based on combining the results of multi-analytical studies together with the mathematical modelling should be integrated. Moreover, the development of the database platforms for the available sensing technologies should be promoted for further progress in biosensorics.
Netz Arroyo - Keynote Presentation
- Feedback Control of Plasma Drug Levels Supported by Continuous In-Vivo Measurements
- Netz Arroyo, Assistant Professor of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, United States of America
Personalized drug therapy seeks to tailor treatment to the individual, taking into consideration each person’s unique genotype and metabolism to determine a dose that maximizes drug efficacy and avoids toxicity. Realizing this goal has been extremely challenging, however, for two main reasons. First, it is difficult to determine the optimal drug dose for each patient due to our limited understanding of pharmacogenetics and physiology. Second, even the most sophisticated drug delivery approaches fail to account for hour-to-hour fluctuations in an individual’s metabolism driven by changes in health status, diet or drug interactions. Thus, there remains a pressing need for technologies supporting the real-time, in-vivo monitoring of drugs that would enable patient-specific, metabolism-responsive dosing. In response to this need, our laboratory pursues the development of reagentless sensing approaches that support continuous measurement in the body. During this talk, I will describe an electrochemical approach that relies on DNA aptamers to perform real-time monitoring of small molecule targets, and the use of this approach to study pharmacokinetic changes in rats that originate from biological and metabolic variability.
- Highly-Refractive Barium Titanate Microspheres For Low-Cost Resolution Enhancement In Optical Imaging of Living Micro-Organisms
- Daniel Migliozzi, Researcher, Ecole Polytechnique Federale de Lausanne, Switzerland
In this work, we performed a systematic study of the increased resolution mediated by highly-refractive microspheres when imaging micro-structures and micro-objects, and we applied this results to the imaging of micro-organisms in aqueous media.
BioDetection and BioSensors Summit 2019 - Speakers
Chair of Nanoengineering, SAIC Endowed Professor, Director at Center of Wearable Sensors, University of California-San Diego
Assistant Professor of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine
Marie-Curie Researcher, Technical University of Denmark
Researcher, Ecole Polytechnique Federale de Lausanne
Professor, Auburn University
Researcher, Technical University of Denmark
Assistant Professor, University of California, San Diego
BioEngineering Summit 2019 - Speakers
Benjamin Mayhugh Associate Professor of Mechanical Engineering, University of Minnesota
Professor of Bioengineering, University of Washington
Professor of Biomedical Engineering and Materials Science & Engineering, Boston University
Associate Professor and Canada Research Chair in Biomedical Engineering, University of Victoria
Researcher, Johns Hopkins University School of Medicine
Director at Translational Center of Tissue Chip Technologies, Massachusetts Institute of Technology (MIT)
Chief Executive Officer, Poietis
Assistant Professor, Cardiac Surgery, Johns Hopkins Hospital
Assistant Professor, Pohang University of Science And Technology (POSTECH)
Senior Research Scientist, Fraunhofer Center for Manufacturing Innovation
Researcher, Ecole Polytechnique Federale de Lausanne
Research Assistant, California Polytechnic State University, San Luis Obispo
- Academic Researchers
- Students and Postdoctoral fellows are encouraged to submit poster abstracts, register for the conference, attend the conference and present their research work in posters - this provides an excellent opportunity for scientific exchange and network with colleagues and fellow researchers in the field
- Scientific researchers from industry as well as business development professionals from industry - given the delegates at these conferences represent a blend of academics and industry participants, these conferences represent an excellent networking opportunity for industry researchers and for licensing and business development professionals from companies
- Vendors offering technologies, tools, products and services to the industry - These conferences provide an excellent venue for vendors that provide innovative technologies and tools to the industry to network and engage with prospective researchers/end-users since these conferences bring together the key opinion leaders (KOLs) in the field.
Coronado Island Marriott Resort & Spa
2000 2nd St
San Diego, CA