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Protocols for Yeast

  • ID: 2179744
  • Book
  • July 2019
  • 450 Pages
  • John Wiley and Sons Ltd
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With its 120 protocols provided in the standard SOP format demanded by many industries, this practical reference covers the whole range of different applications for yeast in both the biotechnology industry and in basic research. In addition, it includes comprehensive tables listing sources and suppliers of the important components found in the protocols, as well as introductory sections on the characteristics of different yeasts. The enclosed CDROM further provides additional protocols not available in the book, electronic versions of the most important protocols and template files for preparing one′s own SOPs.
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Editorial

Introduction to the selected yeast species

Identity testing and storage

Whole cell and organelle analysis

Working with chromosomes, DNA and RNA

Working with proteins

Enzyme tests

Isolation of metabolites

Genetic engineering and analysis of recombinant strains

Generation of cell substrates for approved processes and their testing

Fermentation and process steps

Appendix

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Gerd Gellissen has worked as a Senior Scientist at Rhein Biotech GmbH, Düsseldorf and Associate Professor of Microbiology at the Heinrich–Heine University of Düsseldorf. He has recently founded his own Biotechnology firm in Aachen. He has published approx. 130 research papers and co–authored 7 patents. Professor Gellissen is editor of the Wiley–VCH books "Hansenula polymorpha" (2002) and "Production of Recombinant Proteins" (2004).
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