With its 120 protocols provided in the standard SOP format demanded by many industries, this practical reference covers the whole range of different applications for yeast in both the biotechnology industry and in basic research. In addition, it includes comprehensive tables listing sources and suppliers of the important components found in the protocols, as well as introductory sections on the characteristics of different yeasts. The enclosed CDROM further provides additional protocols not available in the book, electronic versions of the most important protocols and template files for preparing one′s own SOPs.
Table of Contents
EditorialIntroduction to the selected yeast species
Identity testing and storage
Whole cell and organelle analysis
Working with chromosomes, DNA and RNA
Working with proteins
Enzyme tests
Isolation of metabolites
Genetic engineering and analysis of recombinant strains
Generation of cell substrates for approved processes and their testing
Fermentation and process steps
Appendix