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Protocols for Yeast

  • Book

  • 450 Pages
  • July 2019
  • John Wiley and Sons Ltd
  • ID: 2179744
With its 120 protocols provided in the standard SOP format demanded by many industries, this practical reference covers the whole range of different applications for yeast in both the biotechnology industry and in basic research. In addition, it includes comprehensive tables listing sources and suppliers of the important components found in the protocols, as well as introductory sections on the characteristics of different yeasts. The enclosed CDROM further provides additional protocols not available in the book, electronic versions of the most important protocols and template files for preparing one′s own SOPs.

Table of Contents

Editorial
Introduction to the selected yeast species
Identity testing and storage
Whole cell and organelle analysis
Working with chromosomes, DNA and RNA
Working with proteins
Enzyme tests
Isolation of metabolites
Genetic engineering and analysis of recombinant strains
Generation of cell substrates for approved processes and their testing
Fermentation and process steps
Appendix

Authors

Gerd Gellissen has worked as a Senior Scientist at Rhein Biotech GmbH, Düsseldorf and Associate Professor of Microbiology at the Heinrich–Heine University of Düsseldorf. He has recently founded his own Biotechnology firm in Aachen. He has published approx. 130 research papers and co–authored 7 patents. Professor Gellissen is editor of the Wiley–VCH books "Hansenula polymorpha" (2002) and "Production of Recombinant Proteins" (2004).