Lipidomics. Technologies and Applications

  • ID: 2183393
  • Book
  • 356 Pages
  • John Wiley and Sons Ltd
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Focusing on the practical applications, this user–oriented guide presents current technologies and strategies for systems–level lipid analysis, going beyond basic research to concentrate on commercial uses of lipidomics in biomarker and diagnostic development, as well as within pharmaceutical drug discovery and development.

The editor and authors have experience of the most recent analytical instruments and techniques, allowing them to provide here first–hand practical experience for newcomers to the field. The first half of the book covers current methodologies, ranging from global to targeted lipidomics and shotgun approaches, while the second part discusses the role of lipidomics in biomedical and pharmaceutical research, covering such diverse fields as inflammation, metabolic syndrome, cardiovascular and neurological disease. Both small and large–scale, high–throughput approaches are discussed, resulting in an invaluable source for academic and industrial research and development.
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PREFACE

LIPIDOMICS PERSPECTIVE: FROM MOLECULAR LIPIDOMICS TO VALIDATED CLINICAL DIAGNOSTICS

Introduction

Hierarchical Categorization of the Analytical Lipid Outputs

The Type of Lipid Information Delivers Different Biological Knowledge

Untying New Biological Evidences through Molecular Lipidomic Applications

Molecular Lipidomics Approaches Clinical Diagnostics

Current Roadblocks in Lipidomics

Conclusions

LIPIDS IN CELLS

Introduction

Basis of Cellular Lipid Distribution

Lipid Distribution by Nonvesicular Routes

Lipids in Different Cell Types

Functional Implications of Membrane Lipid Composition

Outlook: Collectives and Phase Separation

HIGH–THROUGHPUT MOLECULAR LIPIDOMICS

Introduction

Lipid Diversity

Function of Molecular Lipids

Automated Sample Preparation

Different Approaches to Molecular Lipidomics

Data Processing and Evaluation

Lipidomic Workflows

Conclusions and Future Perspectives

MULTIDIMENSIONAL MASS SPECTROMETRY–BASED SHOTGUN LIPIDOMICS

Introduction

Multidimensional Mass Spectrometry–Based Shotgun Lipidomics

Application of Multidimensional Mass Spectrometry–Based Shotgun Lipidomics for Lipidomic Analysis

Conclusions

TARGETED LIPIDOMICS: SPHINGOLIPIDOMICS

Introduction

Sphingolipids Description and Nomenclature

Sphingolipids Analysis via Targeted LC–MS/MS

Applications of Sphingolipidomics in Biology and Disease

Conclusions

STRUCTURAL LIPIDOMICS

Introduction

Lipid Structure

Structural Analysis of Lipids by Mass Spectrometry

sn Position

Double Bond Position

Double Bond Stereochemistry

Conclusions

IMAGING LIPIDS IN TISSUES BY MATRIX–ASSISTED LASER DESORPTION/IONIZATION MASS SPECTROMETRY

Introduction

Sample Preparation

Matrix

Instrumentation

Data Processing

Conclusions

LIPID INFORMATICS: FROM A MASS SPECTRUM TO INTERACTOMICS

Introduction

Lipid Nomenclature

Basic Properties of Lipid Mass Spectrometric Data

Data Processing

Lipidomic Data Mining and Visualization

Lipidomic Data Integration

Conclusions and Future Perspectives

LIPIDS IN HUMAN DISEASES

Introduction

Obesity

Dyslipidemia

Diabetes

Cardiovascular Disorders

Hereditary Sensory Neuropathy

Neurodegeneration

Cancer

Lysosomal Storage Disorders

Cystic Fibrosis

Anti–Inflammatory Lipid Mediators

Conclusions

LIPIDOMICS IN LIPOPROTEIN BIOLOGY

Introduction

Metabolism of Lipoproteins

Lipoproteinomics in Normolipidemic Subjects

Altered Lipoproteinomics in Dyslipidemia

Conclusions

MEDIATOR LIPIDOMICS IN INFLAMMATION RESEARCH

Introduction

PUFA–Derived Lipid Mediators: Formation and Action

LC–ESI–MS/MS–Based Lipidomics

Mediator Lipidomics in Inflammation and Resolution

Conclusion and Future Perspective

LIPIDOMICS FOR ELUCIDATION OF METABOLIC SYNDROME AND RELATED LIPID METABOLIC DISORDER

Introduction

Basic Strategy of Lipidomics for Elucidating Metabolic Changes of Lipids at the Level of their Molecular Species in Metabolic Syndrome and Related Diseases

Analytical Systems by Mass Spectrometry in Lipidomics

Lipidomic Data Processing ü

Analysis of Lipids as Markers of Metabolic Syndrome

Direct Detection of Lipid Molecular Species in Specific Tissue Domains by Disease–Specific Changes

Conclusions

LIPIDOMICS IN ATHEROSCLEROTIC VASCULAR DISEASE

Introduction

Lipids and Atherosclerotic Vascular Disease

Diagnostics and Treatment

Conclusions

LIPID METABOLISM IN NEURODEGENERATIVE DISEASES

Introduction

Parkinson?s Disease

Conclusions

THE TUMOR MITOCHONDRIAL LIPIDOME AND RESPIRATORY BIOENERGETIC INSUFFICIENCY

Introduction

Cardiolipin and Electron Transport Chain Abnormalities in Mouse Brain Tumor Mitochondria

Complicating Influence of the in vitro Growth Environment on Cardiolipin Composition and Energy Metabolism

Bioinformatic Methods to Interpret Alterations in the Mitochondrial Lipidome

Conclusions

LIPIDOMICS FOR PHARMACEUTICAL RESEARCH

Introduction

Biomarkers for Pharmaceutical Research

Strategy for Biomarker Discovery

Conclusions

INDEX

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Kim Ekroos
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