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Centrosome and Centriole, Vol 129. Methods in Cell Biology

  • ID: 3148992
  • Book
  • July 2015
  • 434 Pages
  • Elsevier Science and Technology
This new volume of Methods in Cell Biology looks at methods for analyzing centrosomes and centrioles. Chapters cover such topics as methods to analyze centrosomes, centriole biogenesis and function in multi-ciliated cells, laser manipulation of centrosomes or CLEM, analysis of centrosomes in human cancers and tissues, proximity interaction techniques to study centrosomes, and genome engineering for creating conditional alleles in human cells.

- Covers sections on model systems and functional studies, imaging-based approaches and emerging studies- Chapters are written by experts in the field- Cutting-edge material

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1. Correlative Light and Electron Microscopy Analysis of the Centrosome; A Step by Step Protocol.
Dong Kong and Jadranka Loncarek
2.Generation of a Conditional Analog-Sensitive Kinase in Human Cells Using CRISPR/Cas9-Mediated Genome Engineering
Tyler Moyer and Andrew J. Holland
3. Studying Centrosome Function Using Three-Dimensional Cell Cultures
Teresa Arnandis and Susana A. Godinho
4. Analysis of Centrosomes in Human Cancer
Stefan Duensing
5. In Vivo Examination of the Cortical Cytoskeleton in Multiciliated Cells Using Electron Tomography
Daniel K. Clare, Maud Dumoux and Delphine Delacour
6.Tackling Centrosome Biology Through Gene Targeting in Chicken B Cells
Pavithra L. Chavali and Fanni Gergely
7. Centriole Biogenesis and Function in Multi-Ciliated Cells
Siwei Zhang and Brian J. Mitchell
8. Sub-Diffraction Resolution Microscopy Methods for Analyzing Centrosomes Organization
Vito Mennella, Rachel Hanna and Moshe Kim
9. Probing Mammalian Centrosome Structure Using Bioid Proximity-Dependent Biotinylation
Elif Nur Firat-Karalar and Tim Stearns
10. Purification of Centrosomes from Mammalian Cell Lines
Delphine Gogendeau, Paul Guichard and Anne-Marie Tassin
11. Isolation, Cryo-Tomography and Three-Dimensional Reconstruction of Centrioles
Paul Guichard, Virginie Hamel, Aitana Neves and Pierre Gönczy
12. New Insights into Centrosome Imaging in Drosophila and Mouse Neuroepithelial Tissues
Maria A. Rujano, Renata Basto and Véronique Marthiens
13. Microinjection Techniques for Studying Centrosome Function in Drosophila Melanogaster Syncytial Embryos
Paul T. Conduit, Daniel Hayward and James G. Wakefield
14. A Yeast Two-Hybrid Approach For Probing Protein-Protein Interactions at the Centrosome
Brian J. Galletta1 and Nasser M. Rusan
15. Genome-Wide RNAi Screens in S2 Cells To Identify Centrosome Components
Jeroen Dobbelaere
16. Time- Lapse Recording of Centrosomes and Other Organelles in Drosophila Neuroblasts
Judit Pampalona, Jens Januschke, Paula Sampaio, and Cayetano Gonzalez
17. Centrosomes and Spindles in Ascidian Embryos and Eggs
Alex McDougall, Janet Chenevert, Gerard Pruliere, Vlad Costache, Celine Hebras, Gregory Salez and Remi Dumollard
18. Ultrastructural Analysis of C. elegans Cilia
Daniel Serwas and Alexander Dammermann
19. Method: In Vitro Analysis of Pericentriolar Material Assembly
Jeffrey B. Woodruff and Anthony A. Hyman
20. Monitoring SPB Biogenesis in Fission Yeast with High Resolution and Quantitative Fluorescent Microscopy
Imène B. Bouhlel, Kathleen Scheffler, Phong T. Tran and Anne Paoletti
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