Mass Spectrometry: Techniques for the Structural Characterization of Glycans presents new methods for conducting detailed carbohydrate qualitative analysis-arming analytical chemists, pharmaceutical scientists, and food scientists with a quick reference that will allow them to determine the structures of carbohydrates molecules.
As there is a need in the scientific community for content specific to structural determination and analysis of new glycoprotein drug, and because structure-activity analysis requires a structural determination of the N- and O-linked oligosaccharides linked to glycol-proteins, this book provides the relevant research that are necessary for advances and new outcomes in this area of study.
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Table of Contents
. Introduction. Monosaccharide Composition Analysis
. Elemental Analysis of MS Ions
. Derivatization Techniques
. Hydride Addition to Sulfate and phosphate- Chemistry
. Instrumentation
MALDI-TOF MS
API 2000 MS
ICP ESI TOF MS
. Hydride Insertion Addition Reaction for the Derivatization of Phosphorylated or Sulfated Glycans and the Discernment of Phosphate versus Sulfate Esters
. Ester Substitution MS Method
The Protocol for the Hydride Addition to the ethanol extract of banana fruit
Example of MS2on Hydride added to the ethanol Extract of Banana Fruit Glycan
. Glycan Isolation Methods
. Other Glycan isolation Methods
. Monosaccharide Analysis
Acid Hydrolysis
. Mass Spectral Carbohydrate Structure Determination Method
MS
MS/MS (MS2)
MSn
Proposing Initial Structure
Refining Methods for Determining Exact Mass Structure
MS Instrumentation Requirements for Discovering Whole Molecule Ions
Derivatization Method and Sulfate/Phosphate Discernment Method
. References
