Separation Science and Proteomics: Current Trends and New Approaches provides readers with the most advanced theories and methodologies on the most important separation methods contributing to current proteomics. Intended to be a practical reference that meets the needs of everyday practitioners, the book also serves as a go-to guide for scientists focused on the theory of separation science, new method development, and new equipment design. Authored by a chemist who has more than 20 years of experience in separation science and proteomics, this book offers readers insights and solutions to the separation mechanisms of a full range of related techniques and complex problems.
Topics covered include the concentration distribution of analyzed protein during IEF separation in natural or IPG gradients and detailed mechanisms of electrophoresis in thermal gradients.
Table of Contents
1. Basic Separation Techniques in Protein Fractionation2. Acid-base equilibria and protein behavior in electrically driven separations
3. Non-aqueous media
4. Electroosmosis
5. Detection Methods
6. Multi-dimensional maps and multistep separations
7. Non-conventional electrophoretic approaches to protein fractionation
8. Sample preparation
9. Mass Spectrometry
10. Proteomics technologies
11. Biophysical Methods contributing to Proteomics
