Mechanisms of DNA Recombination and Genome Rearrangements: Intersection between Homologous Recombination, DNA Replication and DNA Repair, Volume 601, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.
Homologous genetic recombination remains the most enigmatic process in DNA metabolism. The molecular machines of recombination preserve the integrity of the genetic material in all organisms and generate genetic diversity in evolution. The same molecular machines that support genetic integrity by orchestrating accurate repair of the most deleterious DNA lesions, however, also promote survival of cancerous cells and emergence of radiation and chemotherapy resistance. This two-volume set offers a comprehensive set of cutting edge methods to study various aspects of homologous recombination and cellular processes that utilize the enzymatic machinery of recombination. The chapters are written by the leading researches and cover a broad range of topics from the basic molecular mechanisms of recombinational proteins and enzymes to emerging cellular techniques and drug discovery efforts.
- contributions by the leading experts in the field of DNA repair, recombination, replication and genome stability
- documents cutting edge methods
1. Using the Flp recombinase to induce site-specific nicks Lotte Bjergbaek 2. Synthesis from D-loops Wolf-Dietrich Heyer 3. Bacteriophage T4 system as a model for recombination/replication interface Scott Morrical 4. Inducing and detecting mitotic DNA synthesis at difficult-to-replicate loci Ian Hickson 5. Reporter system to study the RNA-DNA recombination Francesca Storici 6. Assaying recombination and mutagenesis at DNA nicks Nancy Maizels 7. Recovery of alternative end-joining repair products from Drosophila embryos Mitch McVey 8. High-throughput analysis of DNA break-induced chromosome rearrangements by amplicon sequencing Steven Roberts 9. Analysis of mutagenesis associated with recombination Jim Haber 10. Investigation of break-induced replication in yeast Anna Malkova 11. Dissecting the dual endo- and exonuclease activity of MRE11 (MRN) with small molecules John Tainer and Davide Moiani 12. RAD52 inhibition Ashley Spies 13. Structure-guided, fragment-based drug discovery against RAD51 recombinase: targeting a protein-peptide interface Thomas Blundell 14. Molecular intermediates of recombination in meiosis (methods, like 2D, and others) Neil Hunter 15. S1-seq method for mapping DSB resection endpoints Scott Keney 16. Methods for studying the roles of the Sgs1-Top3-Rmi1 helicase/decatenase complex in meiotic recombination intermediate metabolism Michael Lichten 17. Live cell imaging of heterochromatin repair in Drosophila cells Irene Chiolo 18. Analysis of meiotic double-strand break initiation in mammals Galina Petukhova and Dan Camerini-Otero