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Field Flow Fractionation. Principles and Applications

  • ID: 4470757
  • Book
  • February 2019
  • 420 Pages
  • John Wiley and Sons Ltd
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Comprehensive and up–to–date, here experienced and outstanding international experts offer a one–stop reference on the principles and applications of this highly versatile separation technology.

Following introductory chapters on the theory, basic principles and instrumentation, a tutorial–style user guide addresses typical users′ questions and problems. In the remaining part, the separation of all relevant particle classes is discussed, covering the latest developments in instrumentation as well as applications.

As a result, both beginners and experienced researchers can confidently rely on this book to confirm their own understanding or to improve their field–flow fractionation methods and the interpretation of the results.
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Chapter 1. The Basics

FFF Overview

Historical perspectives

Normal vs steric vs hyperlayer modes


Resolution, fractionation power

Chapter 2. FFF techniques

AF4 –

Flow FFF (asymmetric, frit inlet and outlet)

HF5– Hollow–fiber flow FFF

Sedimentation FFF

Thermal FFF

Electrical FFF

Novel techniques (including 2D–FFF)

Chapter 3. User guide

Which FFF is right for you?

How to use FF correctly?

General optimization strategies: carrier fluid, calibration, reproducibility

Sample overloading

Sample recovery

Which detector to choose?

How to troubleshoot FFF? (in general terms)

Chapter 4. Other physical fractionation techniques: focus on performance as compared to the FFF



Size exclusion chromatography

Liquid chromatography




Chapter 5. Polymer and latex

Chapter 6. Biological particles: Virus, DNA/RNA, drug delivery

Chapter 7. Nanoparticles in water and soil

Chapter 8. Manufactured nanoparticles

Chapter 9. Protein, enzymes

Chapter 10. Cell separations – blood and algal cells

Chapter 11. Food industry –


Chapter 12. Pharmaceutical industry – drug delivery

Chapter 13. Disease diagnosis
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Céline Guéguen
Mohammed Baalousha
Kim R. Williams
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