Following introductory chapters on the theory, basic principles and instrumentation, a tutorial–style user guide addresses typical users′ questions and problems. In the remaining part, the separation of all relevant particle classes is discussed, covering the latest developments in instrumentation as well as applications.
As a result, both beginners and experienced researchers can confidently rely on this book to confirm their own understanding or to improve their field–flow fractionation methods and the interpretation of the results.
Chapter 1. The Basics
Normal vs steric vs hyperlayer modes
Resolution, fractionation power
Chapter 2. FFF techniques
Flow FFF (asymmetric, frit inlet and outlet)
HF5– Hollow–fiber flow FFF
Novel techniques (including 2D–FFF)
Chapter 3. User guide
Which FFF is right for you?
How to use FF correctly?
General optimization strategies: carrier fluid, calibration, reproducibility
Which detector to choose?
How to troubleshoot FFF? (in general terms)
Chapter 4. Other physical fractionation techniques: focus on performance as compared to the FFF
Size exclusion chromatography
PART II: APPLICATIONS
Chapter 5. Polymer and latex
Chapter 6. Biological particles: Virus, DNA/RNA, drug delivery
Chapter 7. Nanoparticles in water and soil
Chapter 8. Manufactured nanoparticles
Chapter 9. Protein, enzymes
Chapter 10. Cell separations – blood and algal cells
Chapter 11. Food industry –
Chapter 12. Pharmaceutical industry – drug delivery
Chapter 13. Disease diagnosis