Enzymes in Synthetic Biology, Vol 608. Methods in Enzymology

  • ID: 4483019
  • Book
  • 412 Pages
  • Elsevier Science and Technology
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Volume 608 of the series Methods in Enzymology covers key aspects of enzyme discovery, engineering tools and platforms, and examples of applications in the enzymology of synthetic biology.

  • Detailed methods for laboratory use of enzymes in synthetic biology applications
  • Informative case history examples illustrating how enzyme and metabolic engineering are used to generate new products
  • Emphasises latest developments in laboratory automation for the engineering of biology
  • Covers many aspects of the design, build, test, learn cycle used in synthetic biology
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Enzyme Discovery 1. Enzyme selection, cluster discovery and pathway design

Enzyme Engineering Tools and Platforms 2. Cell free systems for biocatalyst discovery and chemically programmed design of biocatalysts 3. Synthetic biology design and synthesis of new biocatalysts

Enzyme Families: Parts and Platforms for Chemical Diversity 4. Terpene Cyclases (i): platform catalysts with non-natural substrates 5. Terpene Cyclases (ii): in vivo platforms for generating chemical diversity 6. Amine oxidases/imine reductases for synthetic biology and pathway bioengineering 7. Flavin biocatalytic parts for pathway engineering 8. P450s for synthetic biology applications

Genome Engineering for Enzyme Pathways 9. Genome editing for microbial synthetic biology 10. (Automated) yeast genome assembly and implications for large scale pathway assembly

Bioengineered Strains with Designed Enzyme Pathways 11. Halomonas and pathway engineering for bio-plastics production 12. Bioengineered carbon assimilation pathways 13. Monoterpene production platforms 14. Commodity chemicals from engineered polyketide synthases 15. Modular pathway rewiring of yeast for amino acid production

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