Introduction to Electrophysiological Methods and Instrumentation, Second Edition, covers all topics of interest to electrophysiologists, neuroscientists and neurophysiologists, from the reliable penetration of cells, the behavior and function of the equipment, to the mathematical tools available for analyzing data. It discusses the pros and cons of techniques and methods used in electrophysiology and how to avoid pitfalls. Although the basics of electrophysiological techniques remain the principal purpose of this second edition, it now integrates several current developments including, amongst others, automated recording for high throughput screening and multimodal recordings to correlate electrical activity with other physiological parameters that are often collected by optical means. A discussion on signal transducers and relevant aspects of microscopy is also included. Together this book provides the electrophysiologist with the tools needed to understand his or her equipment and the acquisitions and analysis of low-voltage biological signals.
- Introduces possibilities and solutions, along with the problems, pitfalls, and artefacts of equipment and electrodes
- Discusses the particulars of recording from brain tissue slices, oocytes, and planar bilayers
- Describes optical methods pertinent to electrophysiological practice
- Presents the fundamentals of signal processing of analogue signals, spike trains, and single channel recordings as well as procedures for signal recording and processing
- Includes appendices on electrical safety and foundations of useful mathematical tools
2. Electrical and Electronic Circuits; Measurements
3. Electronic Devices
4. Electronic and Electrophysiological Instrumentation
5. Electro(de) Chemistry
6. Volume Conduction: Electric Fields in Electrolyte Solutions
7. The Analysis Toolkit
8. Recording of Electrophysiological Signals
9. Analysis of Electrophysiological Signals
10. Microscopy and Optical Methods in Electrophysiology
Dr. Bretschneider is a neurobiologist, currently as guest researcher at Utrecht University (Utrecht, The Netherlands). His research over the years has focused on the field of sensory physiology of aquatic animals, and teaching on sensory physiology and electrophysiology. His current lab interest is in bat research, with recording methods and instrumentation as a main focus.
de Weille, Jan R.
Dr. de Weille has been an Assistant Professor at the Institut des Neurosciences at the University of Montpellier. His research, carried out at the Universities of Nice and later Montpellier, was centred for many years on the pharmacology of ion currents. He developed interests in molecular biology and fluorescence imaging. He has been a consultant with a cancer pharmacology start-up, Beta-Innov (Paris), until his retirement.