Intrinsically Disordered Proteins, Volume 611, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics of interest, including the Characterization of Structure-Function relationships in the intrinsically disordered protein complexin, Distances, distance distributions, and ensembles of IDPs from single-molecule FRET, Biophysical characterization of disordered protein liquid phases, The Use of Mass Spectrometry to Examine IDPs - Unique Insights and Caveats, Fluorescence Depolarization Kinetics to Study Conformational Preference, Structural Plasticity and Membrane Binding of Intrinsically Disordered Proteins, Characterizing the Function of Intrinsically Disordered Proteins in the Circadian Clock, and more.
- Breadth of experimental approaches and systems that will be covered
- The expertise of the contributors writing the articles
Unique Insights and Caveats Perdita Elizabeth Barran 5. Fluorescence Depolarization Kinetics to Study Conformational Preference, Structural Plasticity and Membrane Binding of Intrinsically Disordered Proteins Samrat Mukhopadhyay 6. Characterizing the Function of Intrinsically Disordered Proteins in the Circadian Clock Jennifer Marie Hurley 7. Characterizing allostery in intrinsically disordered proteins Vincent Hilser 8. The role of disorder in HP1 proteins in chromatin phase separation Geeta Narlikar 9. Characterizing the role of residual helicity in binding of intrinsically disordered proteins Gary Daughdrill 10. The use of carbon-detected NMR to characterize flexible and disordered proteins Scott A. Showalter 11. How fuzzy can paramyxoviral NTAIL complexes be? Monika Fuxreiter and Sonia Longhi 12. Structure-function characterization of large disordered proteins Peter Tompa 13. Characterization of intrinsically disordered proteins in protein complexes Birthe B. Krageland 14. Solvent dependent chain dynamics of disordered proteins using fluorescence correlation spectroscopy experiments in cuvettes Kanchan Garai 15. Quanitative characterization of intrinsically disordered protein dynamics by photoinduced electron transfer (PET) Sara Vaiana 16. Multiscale methods to uncover sequence-encoded driving forces for phase separation in proteins with intrinsically disordered regions Rohit Pappu 17. Probing IDP conformations and dynamics with single-molecule fluorescence Ashok Deniz 18. Characterization of IDP function in the nuclear pore Edward A. Lemke 19. Characterizing the impact of intrinsically disordered proteins on membrane curvature Jeanne Stochowiak 20. Novel materials from intrinsically disordered UBX Sarah Bondos 21. Folding and binding of intrinsically disordered proteins Sarah Shammas 22. Characterization of binding of small molecules to intrinsically disordered proteins Steve Metallo 23. Characterizing the roles of intrinsically disordered proteins in cytoplasmic organization Amy Gladfelter 24. Targeting dynamic amyloid states with small molecules Andrew Miranker