Ubiquitin and Ubiquitin-like Protein Modifiers, Vol 618. Methods in Enzymology

  • ID: 4622088
  • Book
  • 412 Pages
  • Elsevier Science and Technology
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Ubiquitination and Protein Stability - Part A Volume 618, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this updated volume presenting interesting chapter written by an international board of authors. Topics of note in this new release include the Preparation of ubiquitinated nucleosomes with native and non-hydrolyzable linkages, Methods to measure ubiquitin chain length and linkage, Genetic approaches to study the yeast ubiquitin system, Enzymatic preparation of monoubiquitinated proteins, Methods to distinguish the function of ubiquitin in autophagy and the proteasome pathway, the Purification and characterization of enzyme activity of USPs, and much more. 

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in this series on enzymology
  • Updated release includes the latest information on methods to measure ubiquitin chain length and linkage, genetic approaches to study the yeast ubiquitin system, amongst many other timely topics
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1. Preparation of ubiquitinated nucleosomes with native and non-hydrolyzable linkages Cynthia Wolberger 2. Methods to measure ubiquitin chain length and linkage Yasushii Saeki 3. Genetic approaches to study the yeast ubiquitin system David Toczyski 4. Enzymatic preparation of monoubiquitinated proteins Helen Walden 5. Methods to distinguish the function of ubiquitin in autophagy and the proteasome pathway Aaron Ciechanover 6. Purification and characterization of enzyme activity of USPs Titia k. Sixma 7. Evaluation of enzyme activities and structures of DUBs David Komander 8. Structural analysis of JAMM/MPN+ DUBs Chittaranjan Das 9. Biochemical methods to study SUMO conjugation Andrea Pichler 10. Methods to study SUMO dynamics in yeast R. Jürgen Dohmen 11. Approaches to study signaling functions of ISG15 Jon Huibregste 12. Methods to analyze STUbL activity Ron Thomas Hay
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