Ubiquitination and Protein Stability, Vol 619. Methods in Enzymology

  • ID: 4622088
  • Book
  • 412 Pages
  • Elsevier Science and Technology
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Ubiquitination and Protein Stability, Volume 619, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this updated volume presenting interesting chapter written by an international board of authors. Topics of note in this new release include the Preparation of ubiquitinated nucleosomes with native and non-hydrolyzable linkages, Methods to measure ubiquitin chain length and linkage, Genetic approaches to study the yeast ubiquitin system, Enzymatic preparation of monoubiquitinated proteins, Methods to distinguish the function of ubiquitin in autophagy and the proteasome pathway, the Purification and characterization of enzyme activity of USPs, and much more.

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in this series on enzymology
  • Updated release includes the latest information on methods to measure ubiquitin chain length and linkage, genetic approaches to study the yeast ubiquitin system, amongst many other timely topics
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1. Preparation of ubiquitinated nucleosomes with native and non-hydrolyzable linkages Cynthia Wolberger 2. Methods to measure ubiquitin chain length and linkage Yasushii Saeki 3. Genetic approaches to study the yeast ubiquitin system David Toczyski 4. Enzymatic preparation of monoubiquitinated proteins Helen Walden 5. Methods to distinguish the function of ubiquitin in autophagy and the proteasome pathway Aaron Ciechanover 6. Purification and characterization of enzyme activity of USPs Titia k. Sixma 7. Evaluation of enzyme activities and structures of DUBs David Komander 8. Structural analysis of JAMM/MPN+ DUBs Chittaranjan Das 9. Biochemical methods to study SUMO conjugation Andrea Pichler 10. Methods to study SUMO dynamics in yeast R. Jürgen Dohmen 11. Approaches to study signaling functions of ISG15 Jon Huibregste 12. Methods to analyze STUbL activity Ron Thomas Hay 13. Assays of SUMO protease function in mammalian cells Stefan Müller 14. In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylation Byung-Hoon Lee 15. Methods to study proteasome regulatory particle assembly Robb Tomko 16. Native mass spectrometry approaches to study the proteasome Michal Sharon 17. Single-molecule methods to study the ubiquitin-proteasome system Ying Lu 18. Assays for the function of ubiquitin in the mammalian endocytic pathway Simona Polo 19. Measuring parkin and ubiquitin function in mitophagy Keiji Tanaka 20. Assays for protein retrotranslocation in ERAD Randy Hampton 21. Assays for protein quality control at the inner nuclear membrane Christian Schlieker 22. LGG-1 western blotting as a read-out for autophagy in C. elegans Thorsten Hoppe 23. Dissecting the elementary enzymatic activities of Ubc7, a key E2 conjugating enzyme of the ERAD pathway Tommer Ravid 24. Methods to study phosphoribosylated ubiquitin ligation and removal Zhao-Qing Luo 25. Assays for ubiquitin-like protein ligation and proteasome function in archaea Julie Maupin-Furlow 26. Ubiquitin and Related Protein Modifier Systems Huib Ovaa
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