Ubiquitin-dependent Protein Degradation, Vol 619

  • ID: 4720948
  • Book
  • 412 Pages
  • Elsevier Science and Technology
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Ubiquitination and Protein Stability - Part B, Volume 619, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this updated volume presenting interesting chapters written by an international board of authors. Topics of note include chapters on Assays of SUMO protease function in mammalian cells, In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylation, Methods to study proteasome regulatory particle assembly, Native mass spectrometry approaches to study the proteasome, Single-molecule methods to study the ubiquitin-proteasome system, Assays for the function of ubiquitin in the mammalian endocytic pathway, and much more.

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in this series on enzymology
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1. Assays of SUMO protease function in mammalian cellsStefan Müller2. In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylationByung-Hoon Lee3. Methods to study proteasome regulatory particle assemblyRobb Tomko4. Native mass spectrometry approaches to study the proteasomeMichal Sharon5. Single-molecule methods to study the ubiquitin-proteasome systemYing Lu6. Assays for the function of ubiquitin in the mammalian endocytic pathwaySimona Polo7. Measuring parkin and ubiquitin function in mitophagyKeiji Tanaka8. Assays for protein retrotranslocation in ERADRandy Hampton9. Assays for protein quality control at the inner nuclear membraneChristian Schlieker10. LGG-1 western blotting as a read-out for autophagy in C. elegansThorsten Hoppe11. Dissecting the elementary enzymatic activities of Ubc7, a key E2 conjugating enzyme of the ERAD pathwayTommer Ravid12. Methods to study phosphoribosylated ubiquitin ligation and removalZhao-Qing Luo13. Assays for ubiquitin-like protein ligation and proteasome function in archaeaJulie Maupin-Furlow14. Ubiquitin and Related Protein Modifier SystemsHuib Ovaa
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