Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Sensors, Vol 621

  • ID: 4720961
  • Book
  • 412 Pages
  • Elsevier Science and Technology
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Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in this series on enzymology
  • Updated release includes the latest information on methods to measure ubiquitin chain length and linkage, genetic approaches to study the yeast ubiquitin system, amongst many other timely topics
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1. Site-directed ethylation of membrane proteins for measuring conformational transitions in lipid bilayers Gianluigi Veglia 2. Design and Synthesis of Fluorescent Activity Probes for Protein Phosphatases Cliff. I. Stains 3. Utilizing Split-NanoLuc Fragments as Luminescent Probes for Protein Solubility in Living Cells Cliff. I. Stains 4. SH2-domain based s[external URL] for intracellular recognition of sulfo-tyrosine Jiantao Guo 5. DNA-encoded immunoglobulins for detection of parasites Thomas Kodadek 6. An engineered TEV protease
calmodulin fusion based sensor for neuronal calcium recording Scott T. Laughlin 7. Split-enzyme-based novel strategies for calcium recording Scott T. Laughlin 8. Peptide engineering platform technology Weijun Shen 9. High-throughput methods for aptamer discovery and analysis Andrej Luptak 10. PEGylation of proteins and peptides Joshua L. Price 11. Quantifying Drug-Target Engagement in Live Cells Using Sulfonyl Fluoride Chemical Probes Lyn Jones 12. In bulla channel synthesis and functional expression system under applied membrane potentials Shigetoshi Oiki 13. Drop-in-Well Chip for the Droplet Interface Bilayer with built-in electrodes and low background noise Shigetoshi Oiki 14. Engineering protein degradation in mammalian cells Laura Segatori 15. Engineering split proteins for the design of AND gates Laura Segatori 16. Novel sensors for chemical cytometry analysis Nancy Allbritton 17. Synthetic cells to study protein: membrane lipid dynamic interactions Brian Y. Chow 18. Inter-subunit cyclization as an approach to stabilize oligomeric proteins and complexes Xudong Qu 19. Metabolic chemical reporters of post-translational modifications Matthew Pratt 20. Methods for Design, Characterisation and Application of Inducible Gene Expression Systems Naglis Malys
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