Three-Dimensional Electron Microscopy, Vol 152. Methods in Cell Biology

  • ID: 4746015
  • Book
  • 362 Pages
  • Elsevier Science and Technology
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Three-Dimensional Electron Microscopy, Volume 152 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters focusing on FIB-SEM of mouse nervous tissue: fast and slow sample preparation, Serial-section electron microscopy using ATUM - Automated Tape collecting Ultra-Microtome, Software for automated acquisition of electron tomography tilt series, Scanning electron tomography of biological samples embedded in plastic, Cryo-STEM tomography for Biology, CryoCARE: Content-aware denoising of cryo-EM images and tomograms using artificial neural networks, Expedited large-volume 3-D SEM workflows for comparative vertebrate microanatomical imaging, and many other interesting topics.

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Cell Biology series
  • Includes the latest information on the Three-Dimensional Electron Microscopy technique
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1. FIB-SEM of mouse nervous tissue: fast and slow sample preparation Anna Maria Steyer 2. Serial-section electron microscopy using ATUM
Automated Tape collecting Ultra-Microtome Mark Terasaki 3. Software for automated acquisition of electron tomography tilt series Guenter Resch 4. Scanning electron tomography of biological samples embedded in plastic Reinhard Rachel 5. Cryo-STEM tomography for Biology Sharon Grayer Wolf 6. CryoCARE: Content-aware denoising of cryo-EM images and tomograms using artificial neural networks Florian Jug 7. Expedited large-volume 3-D SEM workflows for comparative vertebrate microanatomical imaging Filip Braet 8. Tomography
plastic Gaia Pigino 9. In situ analysis of male meiosis in C. elegans Thomas Müller-Reichert 10. Computational approaches for stitching, aligmnent and artifact removel of serial section data Stephan Saalfeld 11. Serial Block Face Imaging for Volume Electron Microscopy Christopher Guerin 12. Using SBFI and FIB-SEM for 3D EM studies of rare events Christopher Guerin 13. Subtomogram Averaging Misha Mikudrya
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