Bacteriology methods for the study of infectious disease will provide knowledge, understanding and experience of contemporary, robust methodologies for studies into the pathogenicity and virulence of human/animal bacterial pathogens.
This book presents contemporary yet widely utilised methodologies for the study of pathogenicity and virulence in bacterial pathogens of human and/or animal origin. Protocols will be clearly outlined with lists of required equipment and reagents, alongside the underpinning theory. This text will therefore provide undergraduate and postgraduate students with essential practical guidance for dissertation projects that focus on the study of infectious bacterial disease. It is ideal for those studying bacteria which cause infectious diseases but who may not specialise in microbiology. The protocols are designed to provide a basis on which to develop individual project ideas, and can be developed further if required using current literature - a start point for additional literature searches is provided.
- Helps users research dissertations and interdisciplinary research projects
- Presents a valuable resource that enables researchers from diverse backgrounds to undertake research within the field of infectious diseases
- Summarizes protocols that give a fundamental start to research, but are highly adaptable or can be built upon and integrated into other methodologies
- Provides knowledge, understanding and experience of contemporary, robust methodologies for studies into the pathogenicity and virulence of human/animal bacterial pathogens
1. Fundamental skills for infectious disease research 2. Bacterial growth in solid and liquid media 3. Microscopy and staining 4. Antimicrobial testing 5. Cell culture-based infection models 6. Biofilm models 7. Gene expression analysis 8: Screening for common virulence traits 9. Community composition studies 10. Invertebrate infection models
Rowena Jenkins, PhD, is a lecturer in Microbiology and Infectious Disease in the College of Medicine at Swansea University, UK. Her research team are focused on the effect of novel antimicrobial agents on pathogenic bacteria found in clinical infections, particularly chronic infections such as those seen in diabetic foot ulcers and cystic fibrosis lungs infection, and the impact on the microbiome within those conditions. Dr. Jenkins also has an interest in the potential for natural antimicrobial agents to improve the efficacy of conventional antibiotics against antibiotic resistant bacteria and biofilms.
Sarah Maddocks, PhD, is a lecturer in Microbiology at Cardiff Metropolitan University, UK. Her research focuses on understanding the interactions between pathogens and their host during the infection process. The overarching aim of these studies is to understand how pathogens utilise or disrupt host processes and to explore and exploit novel ways of impairing these host-pathogen interactions to facilitate clearance of infection. Dr. Maddocks' research also includes biofilm and co-culture models, genomic and molecular analysis of gene expression to prove clinically relevant hypotheses.