Cellular and Animal Models in Human Genomics Research is an indispensable resource for applying comparative genomics in the annotation of disease-gene associated variants identified by human genomic sequencing. Editors Walz and Young have compiled a thorough overview and effective protocols for the use of cellular and animal modeling methods to turn lists of plausible genes into causative biomarkers by rigorous proof for their role in disease. With chapters written by international experts, this book first addresses fundamental aspects of using cellular and animal models in genetic and genomic studies, and moves on to present in-depth examples of specific models and their utility, including yeast, worms, flies, fish, mice, and large animals. Protocols for properly conducting model studies, genomic technology, modeling candidate genes vs genetic variants, integrative modeling, and utilizing induced pluripotent stem cells and employing CRISPR-Cas9, are also discussed in-depth.
- Provides a thorough, accessible resource that helps researchers and students employ cellular and animal models in their own genetic and genomic studies- Offers guidance on how to effectively interpret the results and significance of genetic and genomic model studies for human health- Features chapters from international experts in the use of specific cellular and animal models, including yeast, worms, flies, fish, mice, and large animals, among other organisms
Introduction to human genetics Stankiewicz, Lupski, Tekin 1. Modelling candidate genes vs genetic variants Walz, Young 2. Disease in a dish: Cellular models to understand human conditions Walz, Goldstein 3. The Yeast, a versatile model system to study human disease Lundblad 4. The worm, how can it help? K. A. Caldwell; G.A. Caldwell 5. Understanding human genetic disease with the fly Reiter 6. The fish as a model for human diseases Badano; Katsantis 7. The mouse model Young, Walz 8. Large animal models of rare genetic disorders Eccles; Sasaki 9. Crisper/Cas technology Gurumurthy 10. Integrative modeling and novel technologies in human genomics Young