Purification and Characterization of Secondary Metabolites. A Laboratory Manual for Analytical and Structural Biochemistry

Table of Contents

1. The Structure and Function of Secondary Metabolites that are Secreted by Bacteria 2. The Reagents, Supplies and Equipment that are Necessary to Grow Cultures of Bacteria in the Laboratory and to Purify Secreted Metabolites 3. Overview of the Methods for Purification of Metabolites that are Secreted by Bacteria 4. Absorption Spectrophotometry: Ultraviolet-visible and Infrared 5. High-performance Liquid Chromatography 6. Mass Spectrometry 7. X-ray Crystallography 8. Nuclear Magnetic Resonance Spectroscopy 9. Exercises in Purifying and Characterizing a Quorum-sensing Signal 10. Exercises in Purifying and Characterizing Iron-chelating Molecules 11. Exercises in Purifying and Characterizing a Chloroplast-targeting Phytotoxin 12. Designing your Own Experiments

Authors

Thomas E. Crowley Department of Mathematics and Natural Sciences, National University, La Jolla, CA, USA. Thomas Crowley studied biochemistry as an undergraduate at the University of Illinois Urbana-Champaign. He then pursued graduate studies in molecular biology in the Division of Biology at the California Institute of Technology. As a graduate student and later during postdoctoral studies he used biochemical and genetic methods to examine the regulation of gene expression and the intracellular localization of proteins during animal development. He has taught courses covering a wide range of chemical and biological topics such as general chemistry, biochemistry, microbiology, cell biology and developmental biology. These courses were taught at Montclair State University in New Jersey, Columbia University in New York City, the University of California San Diego and National University in La Jolla, California. He has authored articles derived from his research in a variety of journals and articles derived from his teaching in Biochemistry and Molecular Biology Education (BAMBED). He is currently an adjunct faculty member in the Department of Mathematics and Natural Sciences at National University and a member of the American Chemical Society.