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Protocols Used in Molecular Biology

  • ID: 4986832
  • Book
  • January 2020
  • 186 Pages
  • Bentham Science Publishers Ltd

Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials, and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the applications of the protocols is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification, and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics, and multidisciplinary projects).

Techniques explained in this reference include:

  • Nucleic Acid (DNA/RNA) isolation
  • Next-generation sequencing through real time PCR
  • Western Blotting
  • 2-D gel electrophoresis
  • Immunohistochemistry
  • Chromatin immunoprecipitation (ChIP)
  • Live cell-culture techniques
  • Golgi Staining

Key Features:

  • Recent laboratory protocols
  • Diverse examples of molecular biology experiments
  • Simple step-by-step presentation of information
  • Special focus on scientific and clinical applications

Protocols used in Molecular Biology is essential reading for academicians, molecular biologists, as well as graduate and undergraduate students studying basic and applied research. Pharmacologists and medical researchers can also benefit from the wide array of techniques presented in the book.

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Chapter 1

  • Isolation of Genomic DNA from Plant Tissues

Chapter 2

  • RNA Isolation Protocol from Cells and Tissues  

Chapter 3

  • Analyzing Gene Expression Through Real-Time PCR while Neo-Tissue Regeneration Using Developed Tissue Constructs

Chapter 4

  • A Modified Western Blot Protocol for Enhanced Sensitivity in the Detection of a Tissue Protein

Chapter 5

  • Immunohistochemistry as an Important Technique in Experimental and Clinical Practices

Chapter 6

  • Protocols for the Detection and Proteome Analysis of the Yellow Mosaic Virus-Infected Soyabean Leaves

Chapter 7

  • 2D-Dige a Powerful Tool for Proteome Analysis

Chapter 8

  • Molecular Techniques for Genotyping

Chapter 9

  • Sodium Bisulfite Conversion of Human Genome for DNA Methylation Studies

Chapter 10

  • Chromatin Immunoprecipitation (Chip)  

Chapter 11

  • Osteosarcoma Cell Culture and Maintenance to Detect the Apoptotic Effect of Some Promising Compounds by Potent Markers Viz. DNA Fragmentation and Caspase-3 Activation

Chapter 12

  • Culture and Maintenance of Human Ovarian Carcinoma Cells for Scrutinizing Anti-Cancerous Activities of Various Compounds Via Some Potent Molecular Markers

Chapter 13

  • Dictyostelium Discoideum: Live Cell Imaging in Changing Perspective

Chapter 14

  • The Recent Advancement in Rapid Golgi Method and Result Interpretation

Subject Index

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  • Sandeep Singh
  • Dhiraj Kumar
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