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Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems Part D, Vol 641. Methods in Enzymology

  • ID: 4991108
  • Book
  • August 2020
  • Region: Global
  • 412 Pages
  • Elsevier Science and Technology
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Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems Part D, Volume 641 in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include caged cyclopropanes with improved tetrazine ligation kinetics, an analysis of metabolically labeled inositol phosphate messengers by NMR, cell-permeant caged inositol pyrophosphates for probing ß-cells, imaging phospholipase D activity with clickable alcohols via transphosphatidylation, fluorescent biorthogonal labeling of class B GPCRs in live cells, near-infrared photoactivatable nitric oxide donors with integrated photoacoustic monitoring, and much more.

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on retinoid signaling pathways
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1. Caged cyclopropenes with improved tetrazine ligation kinetics Scott T. Laughlin 2. Analysis of metabolically labeled inositol phosphate messengers by NMR Dorothea Fiedler 3. Cell-permeant caged inositol pyrophosphates for probing ß-cells Carsten Schultz 4. Imaging Phospholipase D Activity with Clickable Alcohols via Transphosphatidylation Jeremy M. Baskin 5. Fluorescent Bioorthogonal Labeling of Class B GPCRs in Live Cells Qing Lin 6. Near-infrared photoactivatable nitric oxide donors with integrated photoacoustic monitoring Jefferson Chan 7. H2S Donors with Optical Responses Michael D. Pluth 8. Visualization of oxidative stress-induced carbonylation in live mammalian cells Susan Bane 9. Highly selective dual sensing of ATP and ADP using fluorescent ribonucleopeptide sensors Takashi Morii 10. Synthesis and applications of Cy5B and maybe joint imaging with Marcus Sauer Martin Schnermann 11. Cell Penetration Profiling Using Chloroalkane Chemical Tools Joshua Kritzer 12. Non-Peptidic Cell-Penetrating Motifs for Mitochondrion-Specific Cargo Delivery Dehua Pei 13. Photocaged DNA Nanocapsule for Cell Delivery Hiroshi Sugiyama 14. A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells Amy Elizabeth Palmer 15. RNA-TAG for covalent labeling of RNA Neal Devaraj 16. Chemical probing of RNA Structure with LASER Robert Spitale 17. Deploying split RNA polymerases to encode protein-protein interactions in RNA in live cells Bryan C. Dickinson 18. Intercalation-enhanced "click" crosslinking of DNA Nathan Luedtke 19. Click chemistry-based amplification and detection of endogenous RNA and DNA molecules in situ using clampFISH probes Sara Rouhanifard
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