Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods, Volume 641 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include caged cyclopropanes with improved tetrazine ligation kinetics, an analysis of metabolically labeled inositol phosphate messengers by NMR, cell-permeant caged inositol pyrophosphates for probing �-cells, imaging phospholipase D activity with clickable alcohols via transphosphatidylation, fluorescent biorthogonal labeling of class B GPCRs in live cells, near-infrared photoactivatable nitric oxide donors with integrated photoacoustic monitoring, and much more.
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Table of Contents
- Enzyme- or light-triggered cyclopropenes for bioorthogonal ligationTing Jiang and Scott T. Laughlin
- Analysis of metabolically labeled inositol phosphate messengers by NMR
Robert Puschmann, Robert K. Harmel and Dorothea Fiedler
- Photo-releasable derivatives of inositol pyrophosphates
Sebastian Hauke, Tamara Bittner, Henning J. Jessen and Carsten Schultz
- IMPACT: Imaging phospholipase D activity with clickable alcohols via transphosphatidylation
Timothy W. Bumpus, Dongjun Liang and Jeremy M. Baskin
- Fluorescent bioorthogonal labeling of class B GPCRs in live cells
Srikanth Kumar Gangam and Qing Lin
- Near-infrared photoactivatable nitric oxide donors with photoacoustic readout
Effie Y. Zhou, Hailey J. Knox, Christopher J. Reinhardt, Gina Partipilo and Jefferson Chan
- H2S donors with optical responses
Michael D. Pluth, Yu Zhao and Matthew M. Cerda
- Visualization of oxidative stress-induced carbonylation in live mammalian cells
Kamalika Mukherjee, Tak Ian Chio and Susan L. Bane
- Receptor-based fluorescent sensors constructed from ribonucleopeptide
Skun Nakano, Hiroaki Konishi and Takashi Morii
- Conformationally restrained pentamethine cyanines and use in reductive single molecule localization microscopy
Siddharth S. Matikonda, Ralph G?tz, Ryan McLaughlin, Markus Sauer and Martin J. Schnermann
- A near-infrared light-mediated cleavable linker strategy using the heptamethine cyanine chromophore
Michael P. Luciano, Saghar Nourian, Alexander P. Gorka, Roger R. Nani, Tadanobu Nagaya, Hisataka Kobayashi and Martin J. Schnermann
- Quantitative measurement of cytosolic penetration using the chloroalkane penetration assay
Kirsten Deprey and Joshua A. Kritzer
- Cell-penetrating and mitochondrion-targeting molecules
George Appiah Kubi and Dehua Pei
- A photocaged DNA nanocapsule for delivery and manipulation in cells
Yihong Feng, Takeshi Tohgasaki, Yasuyuki Shitomi, Hiroshi Sugiyama and Masayuki Endo
- A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells
Esther Braselmann and Amy E. Palmer
- Enzymatic covalent labeling of RNA with RNA transglycosylation at guanosine (RNA-TAG)
Kayla N. Busby and Neal K. Devaraj
- Assaying RNA solvent accessibility in living cells with LASER
Chao Feng and Robert C. Spitale
- Split T7 RNA polymerase biosensors to study multiprotein interaction dynamics
Jeffrey A. Dewey and Bryan C. Dickinson
- Cross-linking cellular nucleic acids via a target-directing double click reagent
Masayuki Tera and Nathan W. Luedtke
- Click chemistry-based amplification and detection of endogenous RNA and DNA molecules in situ using clampFISH probes
Sepideh Tavakoli, Yifang Liu, Jacob Potts and Sara H. Rouhanifard
- Quantification of protein delivery in live cells using fluorescence correlation spectroscopy
Susan L. Knox, Angela Steinauer, Garrett Alpha-Cobb, Adam Trexler, Elizabeth Rhoades and Alanna Schepartz
