The detection and measurement of the dynamic interactions of proteins within the living cell are critical to the understanding of cell physiology and pathophysiology. The field of molecular imaging of living subjects continues to expand and has seen dramatic advances in chemistry, engineering and biomedical applications. Molecular Imaging, Second Edition provides the first point of entry to the research for all scientists interested in this multi-disciplinary field. Molecular imaging is very diverse: new investigators, collaborators, and students entering this field need an authoritative reference to bring this field together. Editors Brian Ross and Sam Gambhir designed this revision precisely to fill this need.
- The most authoritative and effective resource available in the field, written by over 170 of the leading scientists from around the world who have evaluated and summarized the most important methods, principles, technologies and data within the field
- Concepts illustrated with over 600 color figures and molecular-imaging examples
- This edition packed with innovative science, including: Light sheet fluorescence microscopy (LSFM); Mass Spectrometry Imaging; Combining In vitro and in vivo diagnostics; Raman Imaging; Optogenetics; FDA approval of current imaging agents
PART I: MOLECULAR IMAGING TECHNOLOGI ES 2. Imaging of Structure and Function with PET/CT 3. PET/MRI 4. SPECT and SPECT/CT 5. Principles of Micro X-ray Computed Tomography 6. Small Animal SPECT, SPECT/CT, and SPECT/MRI 7. Instrumentation and Methods to Combine Small Animal PET with Other Imaging Modalities 8. Functional Imaging Using Bioluminescent Markers 9. Optical Multimodality Technologies 10. Fiber Optic Fluorescence Imaging 11. Fluorescence Tomography 12. Endomicroscopy 13. lntravital Microscopy 14. Diffuse Optical Tomography and Spectroscopy 15. Ultrasound 16. Molecular Photoacoustic Tomography 17. Optical Projection Tomography 18. Potential Roles for Retrospective Registration in Molecular Imaging
PART II: CHEMISTRY OF MOLECULAR IMAGING 19. Chemistry of Molecular Imaging: An Overview 20. Radiochemistry of PET 21. Radiochemistry of SPECT: Examples of 99mTc and 1111n Complexes 22. Nanochemistry for Molecular 23. Newer Bioconjugation Methods 24. Targeted Antibodies and Peptides 25. Hyperpolarized 13C Magnetic Resonance Imaging-Principles and Applications 26. Magnetic Resonance Imaging Agents 27. Optical Imaging Agents 28. Ultrasound Contrast Agents 29. Multimodality Agents 30. "Click Chemistry": Applications to Molecular Imaging 31. The "One-Bead-One-Compound" Combinatorial Approach to Identifying Molecular Imaging Probes 32. Chemical Biology Approaches to Molecular Imaging 33. Theranostics: Agents for Diagnosis and Therapy 34. Magnetic Nanoparticles 35. Fluorocarbon Agents for Quantitative Multimodal Molecular Imaging and Targeted Therapeutics 36. Aptamers for Molecular Imaging 37. Nonclinical Product Developmental Strategies, Safety Considerations, and Toxicity Profiles of Medical Imaging and Radiopharmaceuticals Products
PART III: MOLECULAR IMAGING IN CELL AND MOLECULAR BIOLOGY 38. Overview of Molecular and Cell Biology 39. Systems Biology 40. Protein Engineering for Molecular Imaging 41. Phage Display for Imaging Agent Development 42. Molecular Imaging of Gene Therapy 43. Developing Diagnostic and Therapeutic Viral Vectors 44. Cell Voyeurism Using Magnetic Resonance Imaging 45. Tumor Vasculature 46. Imaging Hypoxia 47. Molecular Imaging of Protein-Protein Interactions 48. Fluorescence Readouts of Biochemistry in Live Cells and Organisms 49. Imaging of Signaling Pathways
PART IV: APPLICATIONS OF MOLECULAR IMAGING 50. Molecular and Functional Imaging of the Tumor Microenvironment 51. Novel MR and PET Imaging in the RT Planning and Assessment of Response of Malignant Gliomas 52. PET Diagnosis and Response Monitoring in Oncology 53. Magnetic Resonance Spectroscopy Treatment Response and Detection 54. Diffusion MRI: A Biomarker for Early Cancer Treatment Response Assessment 55. Myocardial Metabolism 56. Congestive Heart Failure 57. Molecular Imaging of Atherosclerosis 58. Thrombosis and Embolism 59. Molecular Imaging of Stem Cells in Myocardial Infarction 60. Central Nervous System Molecular Imaging 61. Neuroreceptor Imaging: Applications, Advances, and Limitations 62. PET and SPECT Imaging of Neurodegelierative Diseases 63. Molecular Imaging of Autoimmune Diseases 64. Rheumatoid Arthritis 65. Autoimmune Diabetes 66. Imaging in Asthma
PART V: MOLECULAR IMAGING IN DRUG EVALUATION 67. Molecular and Functional Imaging in Drug Development 68. PET Imaging in Cancer Clinical Trials 69. Magnetic Resonance Imaging in Clinical Trials 70. Imaging of Gene Therapy: Basis and Clinical Trials
PART VI: OTHER 71. Visualization 72. Quantification of Radiotracer Uptake into Tissue 73. Mining Genomic Data for Molecular Imaging Targets 74. Pharmacokinetic Modeling 75. Cost-Effectiveness Analysis/Economics of Probe Development 76. The Regulatory and Reimbursement Process for Imaging Agents and Devices
Dr. Ross is the Director of the Center for Molecular Imaging at the University of Michigan, which provides state-of-the-art imaging services for the research community and offering a broad range of imaging systems (two IVIS bioluminescent/fluorescent imaging systems, an Agilent 7 Tesla horizontal bore MRI system, a Siemens PET/CT, a Trifoil Imaging SPECT/CT, and a Bruker CT system). As part of CMI, his research helps to develop applications for imaging to allow for investigations of cancer growth and response to therapy including noninvasive detection of molecular signaling events in living tissue.
Gambhir, Sanjiv S.
Dr. Gambhir serves as the Director of the Molecular Imaging Program at Stanford (MIPS) and Canary Center at Stanford for Cancer Early Detection. He has authored over 600 publications, has over 40 patents pending or granted, has been featured on the cover of over 25 journals including the Nature Series, Science, and Science Translational Medicine. He is on the editorial board of several journals including Nano Letters, Nature Clinical Practice Oncology, and Science Translational Medicine. He has mentored post-doctoral fellows and graduate students from diverse disciplines. His research focuses on the development of imaging assays to monitor fundamental cellular/molecular events in living subjects with an emphasis on the detection and management of cancer. A particular interest of his research and lab is early cancer detection including combining in vivo and in vitro diagnostics.