Scientific Principles of Adipose Stem Cells provides readers with in-depth and expert knowledge on adipose stem cells, their developmental biologic origins, foundational research on ASC signaling mechanisms and immunomodulatory properties, and clinical insights into applications in regenerative medicine. Topics covered include basic adipose stem cell developmental biology and mechanisms of regulating self-renewal and activation in the stem cell niche, important methods for isolation and characterizing ASCs, and data on the impact on human demographics (age, sex, BMI) on ASC phenotype. A section devoted to ASC biology, ASCs for stem cell therapy and regenerative medicine, and ASCs in tissue engineering applications are also included.
The book is written for scientists and clinicians who are broadly familiar with stem cells and basic cell biology principles and those seeking advanced information on adipose stem cells.
- Coverage of basic adipose stem cell developmental biology (maturation process during embryogenesis) and mechanisms of regulating self-renewal and activation in the stem cell niche
- Includes important methods for isolation and characterizing ASCs, as well as known data any impact of human demographics (age, sex, BMI) on ASC phenotype
- An entire section dedicated to ASC biology, additional sections will be devoted to ASCs for stem cell therapy and regenerative medicine, as well as ASCs in tissue engineering applications
Part 1. Introduction and Front Matter 1. Historical Review of Adipose Stem Cells: Discovery and Important Concepts
Part 2. Basic Biology of Adipose Stem Cells 2. The Developmental Origins of Adipose Tissue 3. Establishing the Adipose Stem Cell Identity: Characterization Assays and Functional Properties 4. Maintenance of Adipose Stem Cells in the Stem Cell Niche: Molecular Mechanisms Regulating Self-Renewal and Activation 5. Adipose Stem Cells in Beige and Brown Fat 6. Adipose Stem Cells and Aging 7. Adipose Tissue and Stem Cell Secretomes 8. Adipose MUSE Cell Expression of Pluripotency Genes and Plasticity 9. Adipose Mesenchymal Stem Cell Homing Mechanisms after Injury 10. Bioreactors and MPS systems for adipose-based pharmacologic screening
Part 3. Adipose Cell Therapy and Regenerative Medicine 11. Animal Models in Adipose Stem Cell Research 12. Adipose Stem Cells and Donor Demographics: Impact of Anatomic Location, Donor Sex, Race, BMI and Health 13. Adipose Stem Cell Immunomodulatory Properties 14. Clinical Experience with Adipose Tissue enriched with Adipose Stem Cells 15. Adipose Stem Cells for Wound Healing and Fibrosis 16. Oncologic Safety of Adipose Cell Therapy 17. FDA Regulations on Adipose Cell Use in Clinical Trials and Clinical Translation
Part 4. Engineering with Adipose Stem Cell 18. Biomaterial Control of Adipose Stem Cell Differentiation 19. Methods for Genetically Modifying Adipose Stem Cells 20. Adipose Stem Cells for Bone and Cartilage Engineering 21. Hematopoietic Potential of Adipose Stem Cells 22. Adipose Stem Cells for Vascular Engineering 23. Adipose Stem Cells for Peripheral Nerve Engineering
Part 5. Conclusions and Back Matter 24. Future Research in Adipose Stem Cells and Regenerative Medicine
Dr. Rubin is endowed Professor of Plastic Surgery and Professor of Bioengineering, at the University of Pittsburgh with a faculty appointment with the McGowan Institute of Regenerative Medicine (MIRM). Dr. Rubin developed a basic science research program in the biology of adipose-derived stem cells and serves as Co-Director of the Adipose Stem Cell Center at the University of Pittsburgh. Dr. Rubin holds leadership positions in national and international plastic surgery societies, is the principal investigator in an NIH-funded line of research aimed at developing cell based methods for clinical soft tissue reconstruction after cancer therapy and holds two FDA cell therapy IDE protocols and an IND, all as sponsor/ investigator. Dr. Rubin's many scientific leadership positions include past president of the International Society of Adipose Therapeutics and Science and past Chairman of the Plastic Surgery Research Council.
He has received numerous awards for research, including the highly competitive Gingrass Award from the Plastic Surgery Research Council. He is the recipient of the American Association of Plastic Surgeons Academic Scholar Award, and the Plastic Surgery Educational Foundation Award for Outstanding Achievement in Basic and Translational Science. In 2007, Dr. Rubin was selected to receive a Presidential Early Career Award for Scientists and Engineers (PECASE). Dr. Rubin contributes expertise in scientific models assessing the use of adult adipose stem cell therapies for tissue engineering and regenerative applications
Kokai, Lauren Elizabeth
Dr. Kokai is co-director of the Adipose Stem Cell Center at the University of Pittsburgh,
faculty of the McGowan Institute of Regenerative Medicine (MIRM) and research faculty member in the Department of Plastic Surgery, School of Medicine. Dr. Kokai has a broad background in soft tissue engineering with specific training in development of novel biomaterials and drug delivery systems for adipose tissue regeneration and use of mesenchymal stem cells for tissue engineering and cell therapy applications. She has extensive experience in developing bioassays for biologic product development and has led collaborations with industry partners to transition preclinical research into commercialized products. Dr. Kokai is a co-Investigator in an NIH funded line of research aimed at developing cell-based methods for clinical soft tissue reconstruction after cancer therapy.
Marra, Kacey Gribbin
Dr. Marra is an Associate Professor in the Department of Plastic Surgery (Primary), and Department of Bioengineering (Secondary), at the University of Pittsburgh. Dr. Marra's academic training was in the areas of polymer chemistry, biomaterials and tissue engineering. She is the Director of the Plastic Surgery Research Laboratory (since 2002) and a core faculty member in the McGowan Institute for Regenerative Medicine, where she has served as a member of the Executive Committee since 2004. She has a publication record of 114 peer-reviewed publications, 24 reviews, 14 book chapters, and 4 patents, and over 570 abstracts in the areas of nerve regeneration, adipose stem cells, bioreactors, tissue engineering, and wound healing. Her funding includes NIH, NSF, and DoD grants in the area of regenerative medicine (including the Armed Forces Institute for Regenerative Medicine, (AFIRM)). She is the Enabling Technology Core Focus Area Leader in AFIRM II. She was Co-Chair of the 2015 Tissue Engineering and Regenerative Medicine International Society (TERMIS) World Congress, held in Boston.