Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work.
This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.
- Explores a wide range of advanced methods to be applied by researchers in molecular biology and biotechnology
- Features clear, step-by-step instruction for applying the techniques covered
- Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
2. Dos and Don'ts in the Lab
3. Equipment Care
4. Agarose Gel Electrophoresis
6. DNA plasmid isolation using Alkaline Lysis Method from E.coli DH5? cells
7. Restriction digestion of Plasmid DNA
8. Genomic DNA extraction from plant leaves using CTAB method
9. Genomic DNA extraction from Silk Worm using modified phenol: chloroform method (Suzuki et al., 1972)
10. Genomic DNA extraction from Fungal Mycelium using modified Cenis, 1992 method
11. Bacterial transformation protocol using CaCl2
12. Centrifugation- Principle and Types
13. Various Chromatographic techniques
14. Thin Layer Chromatography
15. Gel Filtration/Size Exclusion Chromatography
16. Ion Exchange Chromatography
17. Affinity Chromatography
18. Paper Chromatography
19. Growth of bacterial cultures and preparation of growth curve
20. Polymerase Chain Reaction
21. Blotting/ Hybridization
22. Dot Blot Analysis
24. Standard Operating Procedures (SOPs) for various equipment
Dr. Khalid Zaffar Masoodi, a young scientist working as an Assistant Professor at Division of Plant Biotechnology in Sher-e Kashmir University of Agricultural Sciences and Technology of Kashmir (India) holds Masters and Ph.D in Biotechnology. After completing his Ph.D, he worked in Prof. Zhou Wang's Lab at University of Pittsburgh, PA, USA as Postdoctoral Associate (2010-2014). Dr. Masoodi is a recepient of SERB Early career research award 2016, Travel Award, SBUR, USA 2012, Society of Endocrinology Journal Award 2018 and many more. With a glorious academic career, Dr. Masoodi holds a distinction in qualifying CSIR-UGC net in Life Sciences, DBT-BET JRF and has qualified Graduate aptitude test in Engineering and Sciences (GATE).
His lab (Transcriptomics Laboratory also called as K-Lab inspired by Klenow fragment that revolutionized Biotechnology research) as SKUAST-K is well equipped with Fluorescence imaging facility, RT-PCR, cell culture, Western blotting and many state-of-the-art instruments. His research focus is on multi-dimensional work in Transcriptomics of stress biology (biotic/abiotic) in plants and Cancer biology. Apart form that, he is extensively working on drug discovery from medicinal plants. He has produced high-impact papers published in Oncofene, Molecular Cancer therapeutics, Endocrinology, Journal of Functional Foods, Plos One, and many more, and has over 230 sequences in Genbank. He also deciphered the whole transcriptome of six Kashmiri apple varieties. Currently he has three externally funded projects from SERB which focus on high throughput screening of Medicinal plants for prostate cancer and cisgenic apple. In 2019-20 Dr. Masoodi filed 5 patent applications with the Indian Patent office.
Lone, Sameena Maqbool
Sameena Maqbool Lone did her Bachelors in Horticulture, from SKUAST-K, holding an excellent academic record. Currently an M.Sc. scholar in the Division of Vegetable Science, SKUAST-K, working on Morphological and Molecular genetic divergence studies in carrot (Daucus carota L.) under the co-guidance of Dr. Khalid Z. Masoodi.
Rasool, Rovidha Saba
Rhovida Saba Rasool did her B. Sc. Agriculture from SKUAST-Wadura followed by Masters in Plant Pathology from SKUAST-Kashmir Shalimar. Currently she is pursuing Ph.D from Division of Plant Pathology, SKUAST-Kashmir. She has done commendable work in finding new genes involved in pathogenesis of shot hole in stone fruits through insertional mutagenesis in Thyrostroma carphophilus. She has been a good student holding an excellent academic and extracurricular record and was awarded with PhD merit scholarship by SKUAST-K in 2018-19. Apart from this, Rovidha has taken part in various national-level sports competitions and has won many national and state-level awards in baseball and handball.