Methods in Enzymology, Volume 644, the latest release in this ongoing serial, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Site-directed recombination (SDR) in vivo: a fast and reliable tool to unveil beneficial epistasis, Creation and application of amine oxidase with expanded substrate specificities from porcine kidney D-amino acid oxidase, Methods to assess correlation networks for engineering transketolase, Exploration of Enzyme Diversity by Integrating Bioinformatics with Microfluidics, Engineering lytic polysaccharide monooxygenases (LPMOs), Emulsion-based directed evolution of enzymes in yeast, and much more.
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Table of Contents
1. Engineering lytic polysaccharide monooxygenases (LPMOs)Zarah Forsberg, Anton A. Stepnov, Guro Kruge N?rdal, Geir Klinkenberg and Vincent G. H. Eijsink
2. Strategies for the expression and characterization of artificial myoglobin-based carbene transferases
Daniela M. Carminati, Eric J. Moore and Rudi Fasan
3. Methods for the detection and analysis of dioxygenase catalyzed dihydroxylation in mutant derived libraries
Julian L. Wissner, Wendy Escobedo-Hinojosa, Peter M. Heinemann, Andreas Hunold and Bernhard Hauer
4. Enhancing promiscuous chemistries of a Schiff-base forming enzyme by divergent evolution
Duncan S. Macdonald and Donald Hilvert
5. Glutamine-walking: Creating reactive substrates for transglutaminase-mediated protein labeling
Lukas Deweid, Adem H. Parisien, Kiana Lafontaine, L?a N.C. Rochet, Harald Kolmar and Joelle N. Pelletier
6. Engineering aldolases for asymmetric synthesis
Mikael Widersten
7. Ultra-high throughput screening for novel protease specificities
Eirini Rousounelou, Steven Schmitt, Luzius Pestalozzi, Martin Held, Tania M. Roberts and Sven Panke
8. Directed evolution of transcriptional switches using dual-selector systems
Yuki Kimura and Daisuke Umeno
9. Methods for competitive enrichment and evaluation of superior DNA ligases
Janine K. Sharma, Luke J. Stevenson, Katherine J. Robins, Adele K. Williamson, Wayne M. Patrick and David F. Ackerley
10. Directed evolution of custom polymerases using droplet microfluidics
Derek Vallejo, Ali Nikoomanzar and John C. Chaput
11. Optically guided mass spectrometry to screen microbial colonies for directed enzyme evolution
Pu Xue, Tong Si and Huimin Zhao
12. Split enzymes: Design principles and strategy
Shion A. Lim and James A. Wells
